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Veterinary Medicine International
Volume 2015 (2015), Article ID 236436, 7 pages
http://dx.doi.org/10.1155/2015/236436
Research Article

Histomorphometric Evaluation of Superovulation Effect on Follicular Development after Autologous Ovarian Transplantation in Mice

1Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, P.O. Box 1731-71345, Shiraz, Iran
3Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, P.O. Box 1731-71345, Shiraz, Iran
4Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran
5Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

Received 14 September 2015; Revised 8 November 2015; Accepted 10 November 2015

Academic Editor: Sumanta Nandi

Copyright © 2015 Amin Tamadon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effect of superovulation by pregnant mare serum gonadotropin (PMSG) on autologous transplanted ovaries in the lumbar muscles of mice was histomorphometrically evaluated using the indices of number and volume of different kind of follicles and volume of corpora lutea, ovary, and stroma. Angiogenesis was observed after mouse ovarian transplantation on days 14 and 21 after ovarian grafting. After transplantation, the total number and volume of primary and secondary follicles reduced, while PMSG superovulation increased the total number and total volume of tertiary follicles and also the ovarian volume after transplantation. Transplantation increased the average size of primary, secondary, and tertiary follicles. Therefore, primary and secondary follicles can survive after autologous transplantation but their reservations diminished by increasing the time of transplantation. However, number of tertiary follicles and their response to superovulation increased over time after transplantation.