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Veterinary Medicine International
Volume 2016, Article ID 3086353, 9 pages
Research Article

Circadian Rhythm and Stress Response in Droppings of Serinus canaria

1Unit of Basic and Applied Bioscience, Faculty of Veterinary Medicine, University of Teramo, Via Renato Balzarini 1, 64100 Teramo, Italy
2Via per Mosciano, No. 96, Giulianova, 64021 Teramo, Italy
3Via Villafranca No. 11, 72100 Brindisi, Italy

Received 3 October 2016; Accepted 9 November 2016

Academic Editor: Francesca Mancianti

Copyright © 2016 Maura Turriani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Serinus canaria is a widespread domestic ornamental songbird, whose limited knowledge of biology make compelling studies aimed to monitor stress. Here, a commercial enzyme immunoassay was adopted to measure immunoreactive corticosterone (CORT) in single Serinus canaria dropping sample, to monitor the daily fecal excretion of CORT in birds bred singly or in-group and to detect the effect promoted by aviary or small transport cage restraint. A robust daily rhythm of CORT was recorded in animals held on short-day light cycle, independent of bred conditions (single or group), which persisted when space availability was modified in single bred animal (transfer in aviary and transport cages). By contrast, a significant change in CORT excretion was recorded when group bred animals are restrained in a smaller cage. The daily rhythm in CORT excretion in response to manipulation showed the greatest response at the beginning of the light period, followed by the absence of the peak usually recorded at the end of the dark phase. These data indicated that EIA could be used as a reliable noninvasive approach to monitor the stress induced by restraint conditions in Serinus canaria.