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Veterinary Medicine International
Volume 2016, Article ID 3478746, 4 pages
http://dx.doi.org/10.1155/2016/3478746
Research Article

Identification of bapA in Strains of Salmonella enterica subsp. enterica Isolated from Wild Animals Kept in Captivity in Sinaloa, Mexico

1Pathology Laboratory, Faculty of Veterinary Medicine and Animal Husbandry, Autonomous University of Sinaloa, Boulevard San Ángel s/n, Fraccionamiento San Benito, 80246 Culiacán, SIN, Mexico
2Enteric Bacteriology Laboratory, Institute of Epidemiological Diagnosis and Reference, Francisco de P. Miranda 177, Lomas de Plateros, Álvaro Obregón, 01480 Mexico City, DF, Mexico

Received 30 January 2016; Accepted 16 May 2016

Academic Editor: Remo Lobetti

Copyright © 2016 Gabriela Silva-Hidalgo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

bapA, previously named stm2689, encodes the BapA protein, which, along with cellulose and fimbriae, constitutes biofilms. Biofilms are communities of microorganisms that grow in a matrix of exopolysaccharides and may adhere to living tissues or inert surfaces. Biofilm formation is associated with the ability to persist in different environments, which contributes to the pathogenicity of several species. We analyzed the presence of bapA in 83 strains belonging to 17 serovars of Salmonella enterica subsp. enterica from wildlife in captivity at Culiacan’s Zoo and Mazatlán’s Aquarium. Each isolate amplified a product of 667 bp, which corresponds to the expected size of the bapA initiator, with no observed variation between different serovars analyzed. bapA gene was found to be highly conserved in Salmonella and can be targeted for the genus-specific detection of this organism from different sources. Since bapA expression improves bacterial proliferation outside of the host and facilitates resistance to disinfectants and desiccation, the survival of Salmonella in natural habitats may be favored. Thus, the risk of bacterial contamination from these animals is increased.