Research Article

Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide

Table 1

Genes and primer sequences used in the study.

Targeted genesPrimer sequences ()

GAPDH, glyceraldehyde-3-phosphate dehydrogenase 6Forward TCCCTGCTTCTACTGGTGCT
Reverse TGACAAAGTGGTCGTTGAGG
NFB, nuclear factor of kappa light polypeptide gene enhancer in B-cells-like 1Forward CGATTTCGATATGGCTGTGA
Reverse CACCTTCTTCAGCTCCTTGG
MMP 13, matrix metallopeptidase 13 (collagenase 3) 7Forward GCATTCAAAAAGGCCTTCAA
Reverse GGAAGCACAAAGTGGCTTTT
ADAMTS 4, metallopeptidase with thrombospondin type 1 motif 4Forward TGTCAGCTTGGTGGTGACTC
Reverse GTTGAAGACATGGCCCAGTT
COL1A1, collagen, type I, alpha 1 11Forward AGCCAGCAGATCGAGAACAT
Reverse CTGGCCACCATACTCGAACT
COL2A1, collagen, type II, alpha 1 6Forward ACGTCCAGATGACCTTCCTG
Reverse GTCCACACCAAATTCCTGCT
COMP, cartilage oligomeric matrix protein 21Forward CCACGTGAATACGGTCACAG
Reverse TAGGAACCAGCGGTAGGATG