Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide

Table 2

General proinflammatory and anabolic gene expression effects of several treatments applied to the synovial membrane explant (SME) groups in this study.

SME group	Relative gene expression in relation to GAPDH						Biological effect
	NFKB	MMP-13	ADAMTS-4	COL1A1	COL2A1	COMP
Control group plus LPS	↑↑	↑↑↑	↑↑	↑↑	↑	↑	Proinflammatory and catabolic effect
25% Lc-PRG supernatant	↓	↓↓↓	↓↓	↓	↓↓	↓↓	Moderate anti-inflammatory but non anabolic effect
50% Lc-PRG supernatant	↓↓↓	↓↓	↓	↓↓	↓↓	↓↓	Intense anti-inflammatory but moderate catabolic effect
25% Lr-PRG supernatant	↓	↓	↓↓	↓	↑	↓↓↓	Slight anti-inflammatory and anabolic effect
50% Lr-PRG supernatant	↓↓↓	↓	↓↓↓	↓↓	↑↑	↑↑↑	Intense anti-inflammatory and intense anabolic effect

This classification was made only comparing the SME of the control group plus LPS and the SMEs groups cultured with both PRG supernatants at two concentrations plus LPS. ↑ = slight increase. ↑↑ = moderate increase. ↑↑↑ = intense increase. ↓ = slight decrease. ↓↓ = moderate decrease. ↓↓↓ = intense decrease.