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Veterinary Medicine International
Volume 2017, Article ID 8967618, 13 pages
Research Article

Assessment of Pasteurella multocida A Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium

1Laboratory of Veterinary Pathology, Universidad de Ciencias Aplicadas y Ambientales, Calle 222 No. 55-37, Bogotá, Colombia
2Faculty of Science, Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Bogotá, Colombia
3Academic Assistant, Veterinary Medicine Program, Universidad de La Salle, Cra. 7 No. 179-03, Bogotá, Colombia
4Laboratory of Cytogenetics and Genotyping of Domestic Animal UGA, Faculty of Veterinary Medicine, National University of Colombia, Bogotá, Colombia
5Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, National University of Colombia, Bogotá, Colombia

Correspondence should be addressed to Carlos Iregui; oc.ude.lanu@ciugeriac

Received 25 September 2016; Revised 6 December 2016; Accepted 29 December 2016; Published 29 January 2017

Academic Editor: Douglas Morck

Copyright © 2017 Carolina Gallego et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The role of the P. multocida lipopolysaccharide (LPS) as a putative adhesin during the early stages of infection with this bacterium in the respiratory epithelium of rabbits was investigated. By light microscopy and double enzyme labeling of nasal septa tissues, the amount of bacteria attached to the respiratory epithelium and the amount of LPS present in goblet cells at different experimental times were estimated. Transmission electron microscopy (TEM) and LPS labeling with colloidal gold particles were also used to determine the exact location of LPS in the cells. Septa that were challenged with LPS of P. multocida and 30 minutes later with P. multocida showed more adherent bacteria and more severe lesions than the other treatments. Free LPS was observed in the lumen of the nasal septum, forming bilamellar structures and adhering to the cilia, microvilli, cytoplasmic membrane, and cytoplasm of epithelial ciliated and goblet cells. The above findings suggest that P. multocida LPS plays an important role in the process of bacterial adhesion and that it has the ability of being internalized into host cells.