Assessment of Pasteurella multocida A Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium

Figure 5

Respiratory epithelium of the nasal septum that was exposed to P. multocida LPS and then to P. multocida 30 min later (Treatment 4). MET and gold-labeling of LPS using a lectin of Limulus polyphemus. Bacterial structures (Bac) were positively labeled on their outer membrane (arrows) and were associated with the cilia (C) of a ciliated epithelial cell (CE); all microorganisms have lost their capsule. Large numbers of colloidal gold particles are visible at the apical cytoplasm of the cell. Cytoplasmic vacuolization (VAC).