Research Article

Scoring System for Lesions Induced by Different Strains of Newcastle Disease Virus in Chicken

Table 4

Appearance of lesions induced by vNDV in different HE-stained tissues from hr 2 till day 6 pi.

Intranasal inoculation of v NDVNumber of tissues in which lesions appeared, out of 3 examined tissues stained with HE

Hour 2 pi(2/3) in trachea.

Hour 4 pi(3/3) in the trachea and (1/3) in the caecal tonsil.

Hour 6 pi(3/3) in the trachea and caecal tonsil.

Hour 12 pi(3/3) trachea and caecal tonsil, (1/3) in proventriculus and spleen.

Day 1 pi(3/3) trachea and caecal tonsil, proventriculus, spleen, (1/3) bursa of Fabricius and liver, (3/3) kidney and intestine, and (1/3) in the lung.

Day 2 pi(3/3) in the trachea, and caecal tonsil, (3/3) proventriculus and spleen, (1/3) in bursa of Fabricius, (3/3) in the liver, kidney, heart, and thymus, (1/3) in the intestine, and (3/3) in the lung.

Day 4 pi(3/3) in the trachea, and caecal tonsil, (1/3) in the brain, (3/3) in proventriculus, spleen, bursa of Fabricius, liver, kidney, heart, thymus, intestine, and lung.

Day 5 pi(3/3) in the trachea, caecal tonsil, (2/3) in the brain, (3/3) in proventriculus, spleen, bursa of Fabricius, liver, kidney, heart, and thymus, intestine and the lung.

Note. Values between brackets before slash indicate the number of a certain tissue in which lesions were detected pi of vNDV in SPF chickens, while values after slash indicate the whole number of a certain tissue collected, stained with HE, and examined. Lesions were not detected in tissues of control group chickens.