Effects of Super Nutritional Hepatic Copper Accumulation on Hepatocyte Health and Oxidative Stress in Dairy Cows

Figure 2

The 3-nitrotyrosine (3NIT (a, b)) and 4-hydroxynonenal (4HNE (c, d)) stained slides for two separate samples centered on the zone 3 hepatocytes where copper accumulates first in the bovine liver. Central vein (CV) is labeled and slides are magnified at 20x. Cow 1 (a, c) had a hepatic copper concentration of 1264.27 μg/g. Cow 2 (b, d) had a hepatic copper concentration of 173.24 μg/g (ref. range: 40-650 μg/g).

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