Comparative Evaluation of T-Cell Immune Response to BTV Infection in Sheep Vaccinated with Pentavalent BTV Vaccine When Compared to Un-Vaccinated Animals
Table 3
Threshold cycle (t) values of BTV specific qPCR results for vaccinated and unvaccinated sheep.
Serial no
Sheep code
Vaccine group∗
Days post challenge (DPC)
BTV challenge serotype
0
3
7
14
21
1
4
VC
No t
No t
38.17
No t
No t
1
2
14
VC
No t
No t
36.5
No t
No t
2
3
24
VC
No t
No t
No t
No t
No t
10
4
34
VC
No t
No t
No t
No t
No t
16
5
44
VC
No t
No t
No t
No t
No t
23
6
3
VC
No t
No t
No t
No t
No t
1
7
13
VC
No t
No t
No t
No t
No t
2
8
23
VC
No t
No t
No t
No t
No t
10
9
33
VC
No t
No t
No t
No t
No t
16
10
43
VC
No t
No t
No t
No t
No t
23
11
5
VC
No t
No t
No t
No t
No t
1
12
15
VC
No t
No t
No t
No t
No t
2
13
51
VC
No t
No t
No t
No t
No t
10
14
35
VC
No t
No t
No t
No t
No t
16
15
45
VC
No t
No t
No t
No t
No t
23
16
9
UVC
No t
No t
22.94
No t
No t
1
17
17
UVC
No t
No t
24.05
26.5
No t
2
18
29
UVC
No t
22.3
28.27
35.42
26.5
10
19
37
UVC
No t
No t
24.55
26.55
28.01
16
20
48
UVC
No t
No t
25.63
No t
No t
23
∗There was significant difference () between vaccinated and unvaccinated animals in their BTV RNA detection analyzed by Scheirer-Ray-Hare technique. Abbreviations: VC = Vaccinated challenged (), UVC = Unvaccinated challenged ().
