A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Table 3

The daily percent decrease in sperm viability, motility, and intactness of the plasma membrane of Kacang goat semen stored for five days at 5°C in skim milk-egg yolk extender with and without the addition of 150 kDa protein isolated from Simmental bull seminal plasma.

Parameters	Fresh to D1	Fresh to D2	Fresh to D3	Fresh to D4	Fresh to D5
Viability (%)
T0	40.50 ± 0.36^Aa	43.61 ± 0.61^Cb	47.51 ± 0.51^Cb	55.71 ± 0.71^Bb	62.61 ± 0.61^Bb
TI	11.85 ± 0.71^Bc	13.56 ± 0.56^Cc	24.41 ± 0.41^Ba	34.61 ± 0.61^Ba	54.71 ± 0.71^Aa
TII	29.90 ± 0.76^Ab	38.66 ± 0.66^Ba	43.46 ± 0.46^Cb	50.76 ± 0.76^Bb	57.61 ± 0.61^Bb
Motility (%)
T0	37.50 ± 0.83^Aa	41.08 ± 0.08^Bb	44.08 ± 0.08^Bc	53.88 ± 0.88^Ba	60.98 ± 0.98^Bb
TI	9.95 ± 0.28^Bc	10.93 ± 0.93^Cc	21.88 ± 0.88^Ba	32.08 ± 0.08^Ba	44.28 ± 0.28^Aa
TII	26.90 ± 0.23^Ab	36.13 ± 0.13^Ba	40.83 ± 0.83^Db	48.23 ± 0.23^Cb	56.08 ± 0.08^Cb
IPM (%)
T0	35.70 ± 0.21^Aa	41.36 ± 0.36^Cb	42.36 ± 0.36^Dc	50.26 ± 0.26^Bb	58.26 ± 0.26^Ba
TI	6.85 ± 0.36^Ac	12.21 ± 0.21^Db	20.36 ± 0.36^Ba	29.26 ± 0.26^Ba	36.36 ± 0.36^Cb
TII	28.90 ± 0.41^Ab	36.31 ± 0.31^Ba	38.21 ± 0.21^Cb	45.41 ± 0.41^Bb	52.41 ± 0.41^Bb

Different capital letter superscripts in the same row and lowercase letter superscripts in the same column indicate significant differences (). SM-EY: skim milk-egg yolk; T0: SM-EY extender without the protein; T1: SM-EY extender +12 μg protein/100 mL extender; T2: SM-EY extender +24 μg protein/100 mL extender.