A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Table 5

The number of motile sperm per ejaculate, average number of doses, and volume (mL) per dose for intracervical insemination using Kacang goat semen stored at 5°C for five days in skim milk-egg yolk extender with and without the addition of 150 kDa protein isolated from Simmental bull seminal plasma. 5°C for five days.

Group	Day 1	Day 2	Day 3	Day 4	Day 5
T0	3975.42 ± 39.95	3697.57 ± 24.97	3441.09 ± 4.99	2603.26 ± 4.99	1996.26 ± 9.99
	(66/0.32)	(62/0.35)	(57/0.37)	(43/0.49)	(33/0.64)
T1	6330.76 ± 34.96	6275.19 ± 4.99	5339.04 ± 84.89	4467.01 ± 39.95	3423.99 ± 14.98
	(106/0.20)	(105/0.20)	(89/0.24)	(74/0.29)	(57/0.38)
T2	4881.65 ± 34.96	4120.76 ± 74.91	3718.95 ± 74.91	3086.30 ± 44.94	2415.18 ± 74.91
	(81/0.26)	(69/0.31)	(62/0.35)	(51/0.42)	(40/0.53)

The number of motile sperm per ejaculate is shown in bold; the number of doses with 60-million motile sperm cells for intracervical insemination/volume (mL) is in brackets. SM-EY: skim milk-egg yolk; T0: SM-EY extender without the protein; T1: SM-EY extender +12 μg protein/100 mL extender; T2: SM-EY extender +24 μg protein/100 mL extender.