Investigation of Dried Blood Spot Cards for Fatty Acid Analysis Using Porcine Blood
Table 1
Fatty acid (% of total fat) profile of liquid whole blood (WB) (n = 5) and dried blood spots (DBS) (n = 5) of 5 pigs with a Mann–Whitney U-test of WB to DBS samples1.
Type
DBS
WB
Pig ID
1
2
3
4
5
Average
52
53
1
2
3
4
5
Average
Fatty acid
Myristic acid (14 : 0)
1.00
1.25
1.35
1.33
0.98
1.18
0.87
0.66
1.25
1.04
1.40
1.39
1.05
1.23
Palmitic acid (16 : 0)
25.75
25.68
25.01
26.24
22.34
25.00
24.14
25.14
25.68
26.10
25.64
27.70
23.49
25.72
Palmitoleic acid (16 : 1w7)
4.44
4.19
4.25
5.10
6.40
4.88
5.92
5.95
4.40
3.99
4.29
4.64
6.57
4.78
Stearic acid (18 : 0)
7.65
9.05
8.63
8.43
7.28
8.21
10.56
9.55
7.94
9.37
8.47
8.66
6.89
8.27
Oleic acid (18 : 1w9)
22.41
20.50
24.56
24.92
22.06
22.89
24.45
26.56
22.98
20.30
24.44
24.11
21.85
22.74
Linoleic acid (18 : 2w6)
27.65
28.49
24.51
23.70
29.47
26.76
25.20
23.82
27.08
28.66
24.30
23.89
29.53
26.69
γ-Linolenic acid (18 : 3w6)
0.29
0.28
0.38
0.27
0.30
0.30
0.30
0.00
0.23
0.26
0.34
0.20
0.33
0.27
Arachidic acid (20 : 0)
0.16
0.19
0.15
0.08
0.00
0.12
0.11
0.00
0.21
0.15
0.12
0.07
0.00
0.11
Paullinic acid (20 : 1w7)
0.11
0.24
0.24
0.12
0.17
0.18
0.15
2.34
0.11
0.21
0.25
0.09
0.21
0.17
α-Linolenic acid (18 : 3w3)
0.53
0.58
0.56
0.51
0.58
0.55
0.50
0.40
0.56
0.51
0.52
0.42
0.59
0.52
Eicosenoic acid (20 : 2w6)
0.27
0.27
0.25
0.15
0.32
0.25
0.29
0.29
0.28
0.26
0.29
0.21
0.23
0.25
h-γ-Linolenic acid (20 : 3w6)
0.35
0.45
0.33
0.38
0.41
0.38
0.32
0.28
0.33
0.40
0.30
0.32
0.36
0.34
Arachidonic acid (20 : 4w6)
6.19
6.12
6.78
5.85
6.90
6.37
4.16
3.39
6.34
6.17
6.93
5.86
6.38
6.34
EPA (20 : 5w3)
0.18
0.20
0.17
0.18
0.16
0.18
0.15
0.22
0.18
0.20
0.15
0.14
0.17
0.17
Behenic acid (22 : 0)
0.31
0.29
0.25
0.41
0.30
0.31a
0.48
0.32
0.03
0.07
0.04
0.08
0.08
0.06b
Adrenic acid (22 : 4w6)
0.49
0.47
0.50
0.52
0.48
0.49a
0.26
0.06
0.52
0.54
0.61
0.55
0.51
0.55b
DPA (Osbond acid) (22 : 5w6)
0.32
0.34
0.32
0.39
0.36
0.35a
0.13
0.08
0.24
0.32
0.26
0.34
0.30
0.29b
DPA (clupanodonic acid) (22 : 5w3)
0.69
0.59
0.60
0.58
0.51
0.59
0.38
0.25
0.56
0.65
0.56
0.61
0.49
0.57
DHA (22 : 6w3)
1.02
0.66
1.02
0.62
0.86
0.84
0.55
0.19
0.98
0.71
0.96
0.62
0.92
0.84
Nervonic acid (24 : 1)
0.19
0.18
0.14
0.20
0.10
0.16a
0.15
0.10
0.11
0.09
0.12
0.10
0.05
0.09b
Saturates
34.87
36.46
35.40
36.50
30.91
34.83
36.15
35.66
35.11
36.73
35.68
37.89
31.52
35.39
Monoenes
22.70
20.91
24.94
25.25
22.33
23.23
25.38
29.04
23.20
20.60
24.81
24.30
22.11
23.00
PUFA
37.98
38.44
35.41
33.15
40.36
37.07
32.55
29.35
37.29
38.68
35.22
33.17
39.80
36.83
HUFA
9.24
8.83
9.71
8.53
9.68
9.20
6.26
4.69
9.14
9.00
9.78
8.44
9.12
9.10
Total w3
2.43
2.03
2.35
1.88
2.11
2.16
1.73
1.42
2.28
2.08
2.19
1.79
2.16
2.10
Total w6
35.55
36.41
33.07
31.27
38.25
34.91
30.66
27.93
35.01
36.60
33.04
31.37
37.64
34.73
Total w9
22.59
20.68
24.70
25.12
22.16
23.05
25.23
26.70
23.09
20.39
24.56
24.21
21.90
22.83
w6/w3
14.65
17.93
14.10
16.60
18.12
16.28
17.75
19.62
15.34
17.60
15.12
17.50
17.41
16.59
Omega-3 HUFA
20.48
16.44
18.37
16.17
15.80
17.45
19.62
18.61
18.83
17.45
17.05
16.25
17.22
17.36
Omega-6 HUFA
79.52
83.56
81.63
83.83
84.20
82.55
80.38
81.39
81.17
82.55
82.95
83.75
82.78
82.64
1Differing superscripts (a, b) in averages columns are significantly different at () for DBS compared to WB. 2Sample represents the 1-year held DBS card at room temperature and is not included in the DBS average. 3Sample represents the 2-year held DBS card at room temperature and is not included in the DBS average.
