Development and Validation of a Novel ELISA for the Specific Detection of Antibodies against Mycobacterium avium Subspecies paratuberculosis Based on a Chimeric Polyprotein

Figure 4

ELISA cross-reactivity in bovine sera infected with Mycobacterium bovis. Corrected OD at 405 nm (OD_405nm sample minus OD_405nm buffer) obtained by the polyprotein-ELISA (a) and PPA-ELISA (b) of the 95 evaluated sera (47 sera from healthy bovines and 35 sera from M. bovis-infected bovines) for the analysis of the cross-reactivity with M. bovis. The dashed line indicates the cutoff point selected by the analysis of ROC curves for each ELISA. Wilcoxon analysis showed significant differences between the PTB-free and TB-infected groups ( < 0.0001).

(a)

(b)