Detection of Methicillin-Resistant Staphylococcus aureus in Clinical and Subclinical Mastitis in Ruminants and Studying the Effect of Novel Green Synthetized Nanoparticles as One of the Alternative Treatments

Table 2

PCR primers used to detect antimicrobial resistance genes in MRSA isolates.

Isolated bacteria	Target genes	Primer sequence (5′-3′)	Size of amplicon (bp)	References
Methicillin-resistant Staphylococcus aureus	tet(M)	F—AGTGGAGCGATTACAGAA; R—CATATGTCCTGGCGTGCTTA	406 bp	[6–11, 27]
	tet(K)	F—GTAGCGACAATAGGTAATAGT; R—GTAGTGACAATAAACCTCCTA	158 bp
	mecA	F—CCTAGTAAAGCTCCGGA; R—CTAGTCCATTCGGTCCA	331 bp
	mecA	F—CCTAGTAAAGCTCCGGAA CCTAGTAAAGCTCCGGAA; R—CTAGTCCATTCGGTCCA	314 bp
	ermB	F—CTATCTGATTGTTGAAGAAGGATT; R—GTTTACTCTTGGTTTAGGATGAAA	142 bp
	blaZ	F—ACTTCAACACCTGCTGCTTTC; R—TGACCACTTTTATCAGCAACC	173–174

1F = forward; R = reverse.