Molecular Characterization of High Mobility Group Box 1a (HMGB1a) Gene in Red-Bellied Pacu, Piaractus brachypomus

Figure 4

Phylogenetic analysis of HMGB1a sequences from fish and higher vertebrates. The phylogenetic tree was built using the neighbor-joining method and 1000 bootstrap. Fish sequences are grouped separated from mammals. Within the teleost clade, a grouping of different orders is observed, each represented by a different color (green = perciformes; pink = cupleiformes; violet = cypriniformes; yellow = characiformes; orange = siluriformes), thus PbHMGB1a is directly related to the characiform species, Piaractus brachypomus (OQ330857) Colossoma macropomum and Pygocentrus nattereri. The numbers at the nodes indicate the bootstrap values. Sequences of HMGB1a correspond to Colossoma macropomum (XP_306416916), Pygocentrus nattereri (XP_017572966) Silurus meridionalis (XP_046717257), Ictalurus punctatus (XP_017344145), Tachysurus fulvidraco (XP_026995128), Megalobrama amblycephala (XP_048035832), Pimephales promelas (XP_039532079), Labeo rohita (ARX80203), Alosa alosa (XP_048084920), Alosa sapidissima (XP_041949179), Clupea harengus (XP_012683898), Thunnus albacares (XP_044227023), Seriola dumerili (XP_022594514), Lates calcarifer (XP_018547270), Morone saxatilis (XP_035522445), Homo sapiens (NP_001300821), Macaca mulatta (NP_001252735), Mus musculus (NP_001300823), Rattus norvegicus (NP_037095), Gallus gallus (XP_040509225), Anas platyrhynchos (XP_027325676), Pseudonaja textilis (XP_026564649), Thamnophis elegans (XP_032075701).