Molecular Characterization of High Mobility Group Box 1a (HMGB1a) Gene in Red-Bellied Pacu, Piaractus brachypomus

Table 1

Sequences of the primers used for the amplification of HMGB1a and EF-1α from Piaractus brachypomus.

Gene	Sequence (5′-3′)		Tm (°C)	Amplicon size (bp)
HMGB1a (ORF)	F	GGG​ACA​TCT​TCA​ACA​TGG​GG	58.23	640
	R	CCA​ATT​TTT​ACT​CAT​CAT​CGT​CCT​C	58.28
HMGB1a (qPCR)	F	CTG​CAG​AGA​GGA​ACA​CAA​GAA	57.88	198
	R	CTC​TTC​TTC​TTC​TCG​CCT​TTG	56.65
EF-1α	F	ACT​GAG​GTC​AAG​TCT​GTG​GA	57.91	110
	R	CCA​CGA​CGG​ATG​TCT​TTA​A	54.96

HMGB1a, high mobility group box 1a; qPCR, quantitative polymerase chain reaction; EL-1α, elongation factor 1 alpha.