Research Article

Electromyography of the External Anal Sphincter during Micturition and Electrophysiological Bulbocavernosus Reflex in Healthy Spayed Female Canines

Table 2

Computations made with a series of 10 EBCR onset latency values (ms).

MethodUntransformed dataBox-Cox transformed dataNonparametric
StandardRobustStandardRobust

N1010101010
Mean18.721.47
Median18.4018.571.471.47
SD2.692.870.060.06
Minimum15.215.21.391.39
Maximum23.523.51.561.56

λ1 coefficient Box-Cox−5.185−5.185
λ2 coefficient Box-Cox−0.484−0.484
P value Anderson–Darling0.6850.923
Symmetry test for robust0.7460.998

Outliers Dixon
Outliers Tukey0000
Suspect data Tukey0000

Lower limit of reference interval12.34a12.07a13.92a13.85a
Upper limit of the reference interval25.10a25.07a27.30a27.44a
90% CI for lower limit9.9812.65
15.1015.59
90% CI for upper limit22.2222.46
27.8134.50

Comments
The sample size is too small (n < 40) to compute a nonparametric reference interval.
Data should be analyzed with the nonparametric method. As an alternative, the robust method with a Box-Cox transformation may be used after checking the symmetry of the distribution.
The robust method with a Box-Cox transformation of the data gives the following reference interval: [13.85; 27.44].
The sample size is too small (n < 20) to compute the CIs for the limits of the reference interval obtained with the robust method with Box-Cox transformation.

aIn agreement with the IFCC-CLSI recommendation.