Evaluation of In-House ELISA for Antirabies Antibodies Detection in Domestic Canine

Table 1

Assay performance of three diagnostic kits for rabies-specific IgG detection based on the ELISA technique.

	Pusvetma (%)		Platelia II (%)		BukTi-Vet (%)
	Mean	95% CI	Mean	95% CI	Mean	95% CI
Sensitivity	82.88	74.57–89.37	94.59	88.61–97.99	98.19	93.64–99.78
Specificity	97.87	88.71–99.95	95.75	85.46–99.48	97.87	88.71–99.95
Accuracy	87.34	81.13–92.09	94.94	90.27–97.79	98.10	94.55–99.61
PPV	98.93	92.96–99.84	98.13	93.11–99.51	99.09	94.00–99.87
NPV	70.77	61.60–78.51	88.24	77.46–94.24	95.83	85.34–98.91
LR+	38.96	5.59–271.32	22.23	5.72–86.34	46.15	6.64–320.91
LR−	0.18	0.12–0.26	0.06	0.03–0.12	0.02	0.01–0.07
AUC	0.90^a	0.85–0.95	0.95^a,b	0.91–0.98	0.98^b	0.95–0.996
CUI +ve	0.82	0.75–0.89	0.93	0.88–0.97	0.97	0.95–0.99
CUI −ve	0.69	0.61–0.77	0.85	0.78–0.91	0.94	0.89–0.98

Note. All data in the table are expressed in percent except those marked with an asterisk (). PPV, positive predictive value; NPV, negative predictive value; LR, likelihood ratio; AUC, area under curve; CUI, clinical utility index. AUC with different alphabet notations showed a very significant difference (). Benchmark of CUI according to Mitchell [19]: excellent utility (≥0.81), good utility (≥0.64), fair utility (≥0.49), poor utility (<0.49), and very poor utility (<0.36).