Evaluation of In-House ELISA for Antirabies Antibodies Detection in Domestic Canine

Table 3

Assay performance of three ELISA kits based on known positive serum from dogs vaccinated with Rabisin®.

	Pusvetma (%)		Platelia II (%)		BukTi-Vet (%)
	Mean	95% CI	Mean	95% CI	Mean	95% CI
Sensitivity	78.18	64.99–88.19	96.36	87.47–99.56	98.18	90.28–99.95
Specificity	97.92	88.93–99.95	95.75	85.46–99.48	95.83	85.75–99.49
Accuracy	87.38	79.38–93.11	96.08	90.26–98.92	97.09	91.72–99.39
PPV	97.73	86.02–99.67	96.36	87.21–99.04	96.43	87.42–99.06
NPV	79.66	70.33–86.61	95.75	85.22–98.87	97.87	86.83–99.69
LR+	37.53	5.37–262.32	22.65	5.83–87.98	23.56	6.07–91.55
LR−	0.22	0.14–0.37	0.04	0.01–0.15	0.02	0.003–0.13
AUC	0.88^a	0.80–0.94	0.96^b	0.90–0.99	0.97^b	0.92–0.99
CUI +ve	0.76	0.65–0.88	0.93	0.87–0.99	0.95	0.89–0.99
CUI −ve	0.78	0.71–0.85	0.92	0.87–0.97	0.94	0.89–0.98

Note. All data in the table are expressed in percent except those marked with an asterisk (). PPV, positive predictive value; NPV, negative predictive value; LR, likelihood ratio; AUC, area under curve; CUI, clinical utility index. AUC with different alphabet notations showed a significant difference (). Benchmark of CUI according to Mitchell [19]: excellent utility (≥0.81), good utility (≥0.64), fair utility (≥0.49), poor utility (<0.49), and very poor utility (<0.36).