Evaluation of In-House ELISA for Antirabies Antibodies Detection in Domestic Canine

Table 6

Interassay agreement among three ELISA kits based on known positive serum from dogs vaccinated with Rabisin®.

Methods	Pusvetma vs. Platelia II		Pusvetma vs. BukTi-Vet		BukTi-Vet vs. Platelia II
	Coeff.	95% CI	Coeff.	95% CI	Coeff.	95% CI
Cohen’s kappa	0.77	0.65–0.89	0.75	0.63–0.88	0.94	0.88–1
Scott’s pi	0.77	0.65–0.89	0.75	0.63–0.88	0.94	0.88–1
Krippendorff’s alpha	0.77	0.65–0.90	0.75	0.63–0.88	0.94	0.88–1
Gwet’s AC1	0.77	0.65–0.90	0.75	0.63–0.88	0.94	0.88–1
Brennan–Prediger	0.77	0.65–0.90	0.75	0.63–0.88	0.94	0.88–1
Percent agreement	0.89	0.82–0.95	0.88	0.81–0.94	0.97	0.94–1

Note. Altman benchmark for agreement coefficients [21]: very good (0.8–1), good (0.6–0.8), moderate (0.4–0.6), fair (0.2–0.4), and poor (<0.2).