Molecular Detection and Multidrug Resistance of Shigella spp. Isolated from Wild Waterfowl and Migratory Birds in Bangladesh

Table 1

Primers used to detect Shigella spp. and their resistance genes from wild waterfowl and migratory birds.

Target genes	Primer sequence (5′–3′)	Amplicon size (bp)	Annealing temperature (°C)	References
Shigellagenus-specific gene	F: AAC​TGG​TTA​CCT​GCC​GTG​AG	154	56	[21]
	R: TGG​TGA​TGG​TGG​TGG​TAA​TG
tetA	F: GGT​TCA​CTC​GAA​CGA​CGT​CA	577	57	[22]
	R: CTG​TCC​GAC​AAG​TTG​CAT​GA
tetB	F: CCT​CAG​CTT​CTC​AAC​GCG​TG	634	56	[22]
	R: GCA​CCT​TGC​TGA​TGA​CTC​TT
SHV	F: TCG​CCT​GTG​TAT​TAT​CTC​CC	768	53	[23]
	R: CGC​AGA​TAA​ATC​ACC​ACA​ATG