Mosaic TP53 Mutation on Tumour Development in Pigs: A Case Study
Table 1
Sequences of the TP53 target region obtained following the analysis of genomic DNA of ear biopsies from pigs A and B.
Pig
Gender
Genotype
Sequence
Indels
Frequency (%)
Total mutation rate (%)
Target
GTAGGTCTTCTGAGAAGGGACAA AGGACGACAGGGGCCAG
A
♂
Mosaic
GTAGGTCTTCTGAGAAGGGACAAAGGACGACAGGGGCCAG
Wt
18875/44723 (42.2%)
24389/44723 (54.5%)
GTAGGTCTTCTGAGAAGGG-CAAAGGACGACAGGGGCCAG
−1 bp
18780/44723 (42.0%)
GTAGGTCTTCTGAGAAGGGAGACCTACAAAGGACGACAGGGGCCAG
+6 bp
2833/44723 (6.3%)
GTAGGTCTTCTGAGAA—GACGACAGGGGCCAG
−9 bp
2776/44723 (6.2%)
B
♂
Biallelic
GTAGGTCTTCTGAGAAG—GGACGACAGGGGCCAG
−7 bp
24942/50232 (49.7%)
48895/50232 (97.3%)
(With in frame)
GTAGGTCTTCTGAGAAGGGA—ACGACAGGGGCCAG
−6 bp
23953/50232 (47.7%)
The nucleotides in bold and italic font represent target sequences and PAM sequences of sgRNA, respectively. The nucleotides in underline represent the inserted sequence. Frequency was determined by deep sequencing analysis. Wt: wild-type.
