Extender Supplementation with Glutathione (GSH) and Taurine Improves In Vitro Sperm Quality and Antioxidant Status of New Zealand Rabbits during Chilled Storage for up to 72 hours

Table 1

Primer sequences and annealing temperature.

Gene	Primer	Annealing	Slope	Efficiency	Reference
SOD	F: CCC​GGT​CTT​TGT​ACT​CTC​GT	60°C + preheating (at 62°C for 1 min)	−3.34	99.25	[46] NM_001082627
	R: AAG​GAT​GAA​GAG​AGG​CAC​GT
CAT	F: GCC​CTG​GTC​AGT​CTT​GTA​GT	62°C	−3.42	96.06	[46] XM_002709045
	R: CCA​TCG​GCA​TAT​GAA​CGG​AT
GAPDH	F: ATC​TCG​CTC​CTG​GAA​GAT​GG	60°C + preheating (at 62°C for 1 min)	−3.39	97.24	[46] NM_001082253
	R: CAA​AGT​GGA​TGT​TGT​CGC​CA

SOD = superoxide dismutase; CAT = catalase; GAPDH = glyceraldehyde-3-phosphate dehydrogenase (reference gene).