Extender Supplementation with Glutathione (GSH) and Taurine Improves In Vitro Sperm Quality and Antioxidant Status of New Zealand Rabbits during Chilled Storage for up to 72 hours

Table 2

Effect of different concentrations of glutathione and taurine supplementation (0.5, 1, and 2 mM GSH and 1, 5, and 10 mM taurine) on rabbit sperm motility, vitality, and acrosome reaction rate during 24, 48, and 72 h storage at 5°C.

	Postchilling time (h)	Sperm motility (%)	Sperm vitality (%)	Acrosome reaction rate (%)
C	24	87.5 ± 0.50^Aa	93.00 ± 2.00^Aa	85.00 ± 0.50^Aa
	48	74 ± 1.00^Bb	84.00 ± 4.00^Bb	67.00 ± 2.00^Ba
	72	50 ± 5.00^Bc	77.50 ± 2.50^Cc	47.50 ± 2.50^Bb
G0.5	24	88 ± 1.00^Aa	93.50 ± 1.50^Aa	88.00 ± 1.00^Aa
	48	77 ± 2.00^Aa	86.50 ± 1.50^Aa	77.50 ± 2.50^ABa
	72	73 ± 2.00^Ab	82.5 ± 2.50^Aa	62.50 ± 2.50^Bb
G1	24	93 ± 2.00^Aa	94.00 ± 1.00^Aa	89.00 ± 2.00^Aa
	48	82.5 ± 2.50^Aa	91.50 ± 1.50^Aa	78.50 ± 6.50^ABa
	72	66 ± 6.00^Aa	90.5 ± 2.50^Aa	67.00 ± 2.00^Aa
G2	24	93 ± 1.00^Aa	94.5 ± 0.50^Aa	93.50 ± 0.50^Aa
	48	87 ± 2.00^Ab	92.00 ± 1.00^Bb	87.00 ± 1.00^ABa
	72	83 ± 3.00^Ab	91.00 ± 2.00^Bb	82.50 ± 2.50^Bb
T1	24	92.5 ± 2.50^Aa	93.00 ± 2.00^Aa	98.50 ± 0.50^Aa
	48	87.5 ± 0.50^Aa	91.50 ± 1.50^Aa	81.00 ± 6.00^Aa
	72	74.5 ± 1.50^Ab	89.50 ± 3.50^Ab	61.00 ± 6.00^Bb
T5	24	93.5 ± 0.50^Aa	92.5 ± 2.50^Aa	89.50 ± 0.50^Aa
	48	77.5 ± 2.50^Ab	92.50 ± 0.50^Aa	83.00 ± 1.00^Aa
	72	73.5 ± 1.50^Ab	91.5 ± 1.50^Aa	72.50 ± 2.50^Aa
T10	24	91.5 ± 0.50^Aa	95.00 ± 0.00^Aa	92.00 ± 1.00^Aa
	48	84 ± 1.00^Aa	94.00 ± 1.00^Aa	88.00 ± 2.00^Aa
	72	79.5 ± 0.50^Aa	91.50 ± 3.50^Aa	82.00 ± 2.50^Aa

Uppercase and lowercase letters indicate statistical significance at  < 0.05 between chilling times and extender supplements (GSH and taurine). C represents the control samples (no treatment), and G1, G2, and G0.5 represent 1, 2, and 0.5 mM of GSH, respectively. T1, T5, and T10 denote 1, 5, and 10 mM of taurine, respectively.