Research Article
Correlation between Post-Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression
Table 1
Genes used for validation and quantification in this study.
| | Gene | Primer sequence | Accession ID | Annealing temperature |
| | OPN | F: 5′-ATGCATGACGCACCTAAGAAG-3′
R: 5′-TCAATTGACCTCAGAAGAGGC-3′ | AY878328 | 53°C |
| | PPIA | F: 5′-ATGCTGGCCCAACACAA-3′
R: 5′-CCCTCTTTCACCTTGCCAAA-3′ | XM_001252921.1 | 55°C |
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