Phenotypic and Cytogenetic Characterization of Mesenchymal Stromal Cells in De Novo Myelodysplastic Syndromes

Figure 2

Expression of positive CD markers of culture-expanded P2 MSCs analyzed by flow cytometry. Results were analyzed according to the subtypes: RCUD (), RCMD (), RAEB (), and controls (). Bar graphs represent the mean % of positive cells ± standard error. The expression profile was categorized as follows: negative: 0–2%, low: 3–20%, medium 21–50%, medium high: 51–80%, and high: >80%. All MSCs isolated from controls and MDS subtypes showed high positivity for CD90. Control: %, RCUD: %, RCMD: %, and RAEB: %. CD73 expression in RCMD was low (%) compared to that of control, RCUD, and RAEB patients (%, %, and %, resp.). CD105 expression was high in RCMD (>92.6 ± 1.2%) compared to that of control (%) and RCUD (%) and RAEB (%).