Table 2: Leucocyte subsets before, during, and after hemodialysis.

Before dialysisAfter one hour of dialysisAt the end of dialysis15 minutes after end of dialysisReference range

Natural killer cells (% of lymphocytes)
Median (min–max)9.1 (3.7-22.1)7.3 (3-19.8)11.5 (3.8-19)11.6 (3.4-21.6)3-22
Natural killer T cells (% of lymphocytes)
Median (min–max)1.9 (0.4-8.6)1.6 (0.1-5.1)1.9 (0.4-5.9)2.1 (0.3-7.1)2.1-13.7
CD4+ T cells (% of lymphocytes)
Median (min–max)50.9 (36.8-66.5)53.8 (39.5-68.8)52.3 (43.2-64.3)51.7 (42.6-64.5)31-51
CD8+ T cells (% of lymphocytes)
Median (min–max)24.1 (13.6-41.4)22.5 (13.5-27.2)20.8 (11.6-33.8)22.2 (11.8-31)18-35
CD4- CD8- T cells (% of T cells)
Median (min–max)4.2 (1.9-7.7)3.7 (1.6-6.3)3.3 (1.5-5.4)3.4 (1.7-6.5)3-10.2
CD25+ T cells (% of T cells)
Median (min–max)37.4 (24.4-55)38.6 (26.9-54.8)40.6 (27.4-59.1)39.5 (26.8-56.4)22.9-44.9
CD4+ HLA-DR+ T cells (% of CD4+ T cells)
Median (min–max)9.1 (2.8-21.7)9.4 (2.8-21.1)8.5 (3.3-27.7)8.3 (3.4-28.4)5-25
CD8+ HLA-DR+ T cells (% of CD8+ T cells)
Median (min–max)23.5 (1.8-55.4)18.6 (1.6-53.3)18 (1.8-59.7)21.9 (1.4-61.4)5-25
Naive CD8+ T cells (% of CD8+ T cells)
Median (min–max)16.5 (8.5-43.7)22.1 (11-53.2)16.6 (6.8-46.4)18.7 (6.8-56.5)28.4-66.7
CD8+ effector cells (% of CD8+ T cells)
Median (min–max)31.2 (15.2-75.3)28.1 (3.7-52.1)29.7 (8.6-62.5)30.9 (10.1-59.3)7.4-24.6
CD8+ CCR5+ effector cells (% of CD8+ T cells)
Median (min–max)9.3 (0.2-29.9)6 (0.7-26)10.4 (1.2-25.6)10.5 (1-31.5)< 5.9
Regulatory T cells (% of T cells)
Median (min–max)6.2 (4.3-9.2)6.9 (4.7-10.1)6.9 (4.6-10)7.2 (4.5-10)2.8-7.2
HLA-DR+ Tregs (% of Tregs)
Median (min–max)25.1 (9.3-38.4)28.2 (9.5-38.5)25.7 (8.6-45.8)26.6 (9.4-46)5.9-18.8
Eosinophils (% of leukocytes)
Median (min–max)4 (1-10)3 (0-7)3 (0-6)2 (0-6)1-3
Neutrophils (% of leukocytes)
Median (min–max)63 (51-77)68 (46-87)68 (52-79)67 (50-87)55-75

Median (min (minimum)–max (maximum)) in percent in fluorescence-activated cell sorting analysis (FACS) except for neutrophil and eosinophil counts (differential blood count by microscopy). Statistical analysis with nonparametric tests are shown in Table 3.