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Applied and Environmental Soil Science
Volume 2011 (2011), Article ID 537459, 10 pages
http://dx.doi.org/10.1155/2011/537459
Research Article

Relationships among Contrasting Measurements of Microbial Dynamics in Pasture and Organic Farm Soils

1Department of Biology, Chatham University, Pittsburgh, PA 15232, USA
2Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506-6108, USA

Received 1 March 2011; Revised 16 May 2011; Accepted 20 May 2011

Academic Editor: M. Miransari

Copyright © 2011 S. L. Edenborn et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Soil bacteria exhibit short-term variations in community structure, providing an indication of anthropogenic disturbances. In this study, microbial biomass carbon (MBC), potentially mineralizable nitrogen (PMN), community level physiological profiling (CLPP), and culture-dependent DGGE (CD DGGE) fingerprinting of the 16S rRNA gene were used to compare microbial communities in organic farm and pasture soils subjected to differing agronomic treatments. Correlation analyses revealed significant relationships between MBC, PMN, and data derived from microbial community analyses. All measures separated soil types but varied in their ability to distinguish among treatments within a soil type. Overall, MBC, PMN, and CLPP were most responsive to compost and manure amendments, while CD DGGE resolved differences in legume cropping and inorganic fertilization. The results support the hypothesis that culturable soil bacteria are a responsive fraction of the total microbial community, sensitive to agronomic perturbations and amenable to further studies aimed at linking community structure with soil functions.