Characterization of Zebrafish von Willebrand Factor Reveals Conservation of Domain Structure, Multimerization, and Intracellular Storage

Table 1

List of primers and sequences.

Reference number	Sequence	Description
92	AGTCGGCAGCACATACACAC	vwf cloning, assembly of fragment 1 (EcoRI-BstBI)
93	ATCCGGACAGGTCAGTTCAC	vwf cloning, assembly of fragment 1 (EcoRI-BstBI)
94	CCTGCAGCTTAAACCCAAAG	vwf cloning, assembly of fragment 2 (BstBI-AvaI)
95	AAAGCTTCATCGTCCAGCTC	vwf cloning, assembly of fragment 2 (BstBI-AvaI)
96	CTGTTGACGGCAAGTGCTAA	vwf cloning, assembly of fragment 3 (AvaI-SbfI)
97	TCTCCTGATGCTGGACACAC	vwf cloning, assembly of fragment 3 (AvaI-SbfI)
98	GACGGCAGTGTAACGACAGA	vwf cloning, assembly of fragment 4 (SbfI-ApaLI)
99	CCTGCAAGAGAGCCGATAAC	vwf cloning, assembly of fragment 4 (SbfI-ApaLI)
116	TGCGTGCTGAATCAAACTGT	vwf cloning, 3′ RACE (ApaLI-SpeI), SpeI vector derived
128	AGTCGCCAGGGAATTCATAA	vwf cloning, 5′ RACE (NotI-EcoRI), NotI vector derived
130	TTTGATTGACATTTTTATTTATTGTAGTTTA	vwf cloning, amplification of 3′ UTR
543	gatttaggtgacactatagCGACATGCAAGTGCAGAAGT	424 bp vwf riboprobe (exon 28) with SP6 promoter overhang
544	taatacgactcactatagggGCTGGGTTTTGCTGTAGGAG	424 bp vwf riboprobe (exon 28) with T7 promoter overhang
545	gatttaggtgacactatagGGAGTTATCGGCTCTCTTGC	441 bp vwf riboprobe (exons 47–52) with SP6 promoter overhang
546	taatacgactcactatagggACACAGACTTGCTGCCACAC	441 bp vwf riboprobe (exons 47–52) with T7 promoter overhang
	CTCGAGAGAATTCCACCACACTGGACTAGTGGATC	XhoI-PmeI human VWF linker sequence (includes Myc/HIS tag)
	CGAGCTCGGTACCAAGCTTGGGCCCGAACAAAAAC
	TCATCTCAGAAGAGGATCTGAATAGCGCCGTCGAC
	CATCATCATCATCATCATTGAGTTTAAAC