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Advances in Materials Science and Engineering
Volume 2013, Article ID 690918, 6 pages
Research Article

The Effect of Ag Content of the Chitosan-Silver Nanoparticle Composite Material on the Structure and Antibacterial Activity

1Department of Chemical Engineering, Istanbul University, Avcilar, 34320 Istanbul, Turkey
2Department of Medical Microbiology, Cerrahpasa Medical Faculty, Istanbul University, Cerrahpasa, 34098 Istanbul, Turkey

Received 11 September 2013; Accepted 26 November 2013

Academic Editor: Jie Dai

Copyright © 2013 Solmaz Akmaz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study is to investigate the antibacterial properties and characterization of chitosan-silver nanoparticle composite materials. Chitosan-silver nanoparticle composite material was synthesized by adding AgNO3 and NaOH solutions to chitosan solution at 95°C. Different concentrations (0,02 M, 0,04 M, and 0,06 M) of AgNO3 were used for synthesis. Chitosan-silver nanoparticle composite materials were characterized by Transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet (UV) spectrophotometer, and Fourier transform infrared (FTIR) spectrometer techniques. Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Streptococcus pneumoniae were used to test the bactericidal efficiency of synthesized chitosan-Ag nanoparticle composite materials. The biological activity was determined by the minimum bacterial concentration (MBC) of the materials. Antibacterial effect of chitosan-silver nanoparticle materials was increased by increasing Ag amount of the composite materials. The presence of small amount of metal nanoparticles in the composite was enough to significantly enhance antibacterial activity as compared with pure chitosan.