Figure 5: Expression levels of type II collagen in MSCs after 1 week culture on polystyrene dish (PS), S-PAAm gel with of ~1, ~15, and ~150 kPa, respectively. Scale bar, 100 μm. Type II collagen was detected with immunofluorescent. In the immunofluorescent staining, the cells were stained with an antitype II collagen antibody (green) and Hoechst 33258 (blue). Reproduced with permission from [23]. © 2010, Elsevier, Inc.