A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats

Figure 3

Histopathological indications of immature coconut fiber (multifilament) sutured skin area sections. (a) 24 hrs—hematoxylin and eosin (H and E) 10x, necrosis of epidermal and dermal cells, mild neutrophilic infiltration. (b) 48 hrs—Giemsa 10x, epithelial cell migration and moderate neutrophilic infiltration. (c) 72 hrs—H and E 10x, moderate epidermal proliferation, mild granulation tissue invasion. (d) 96 hrs—H and E 10x, epidermal layer thickening, moderate granulation tissue formation. (e) 120 hrs—H and E 10x, moderate epidermal keratinization, mild collagen proliferation and granulation tissue. (f) 144 hrs—van Gieson 10x, moderate amount of diffuse collagen deposition with granulation tissue formation. (g) 168 hrs—PAS 10x, moderate amount of new blood vessels with granulation tissue.

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