A Combination of Coconut Fiber Suture and Tamarind Seed Gel with Dehydrated Human Amnion Membrane for Wound Surgery in Rats

Figure 4

Histopathological indications on immature coconut fiber (monofilament) sutured skin area sections. (a) 24 hrs—H and E 10x, necrosis of epidermal and dermal cells with scab formation and severe neutrophilic infiltration. (b) Epithelial cell migration and proliferation with severe neutrophilic infiltration. (c) 72 hrs—H and E 10x, mild epidermal proliferation with granulation tissue invasion. (d) 96 hrs—H and E 10x, epidermal layer thickening, mild granulation tissue formation. (e) 120 hrs—H and E 10x, severe epidermal keratinization, moderate invasion of granulation tissue. (f) 144 hrs—van Gieson 10x, organized collagen proliferation. (g) 168 hrs—PAS 10x, moderate amount of neovascularization, granulation tissue formation.

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