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Advances in Virology
Volume 2011, Article ID 281425, 5 pages
Review Article

Testing Strategies for Detection of Xenotropic Murine Leukemia Virus-Related Virus Infection

Laboratory of Molecular Virology, CBER, Food and Drug Administration, Bethesda, MD 20892, USA

Received 10 March 2011; Revised 18 May 2011; Accepted 10 June 2011

Academic Editor: Myra McClure

Copyright © 2011 Shixing Tang and Indira K. Hewlett. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Xenotropic murine leukemia virus-related virus (XMRV) is a newly identified gamma retrovirus and may be associated with prostate cancer- (PC) and chronic fatigue syndrome (CFS). Since its identification in 2006 and detection of polytropic murine lenkemia virus (MLV)-like sequences in CFS patients in 2010, several test methods including nucleic acid testing methods and serological assays have been developed for detection of XMRV and/or MLV-like sequences. However, these research assays have not yet been validated and evaluated due to the lack of well-characterized reference materials. Mouse DNA contamination should be carefully checked when testing human specimens in order to avoid false-positive detection of XMRV or MLV-like sequences.