BioMed Research International / 2008 / Article / Tab 1

Research Article

Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production

Table 1

Bacterial strains and plasmids used in this study.

Strain or plasmidRelevant characteristicsReference or source

E. coli strains
XL1-BluerecA1, endA1, gyrA96, thi, hsdR17, suppE44, relA1, l-, lac-, F’[proAB laclq lacZ M15, Tn10 (tet)r]Stratagenea
W3110F-mcrA mcrB IN(rrnD-rrnE)1 -KCTCb

Plasmids
pTac99ApTrc99A derivative; tac promoter, cloning vehicle; AprPark and Lee [9]
pAD99ApTac99A derivative; aldehyde dehydrogenase (aldA) promoter; AprThis study
pUP99ApTac99A derivative; uridine phosphorylase (udp) promoter; AprThis study
pGFPuvApr, lac promoter, gfpClontechc
pAD99GFPpAD99A derivative; gfpThis study
pTac99GFPpTac99A derivative; gfpThis study
pUP99GFPpUP99A derivative; gfpThis study

aStratagene Cloning System (La Jolla, Calif, USA).
bKorean Collection for Type Cultures, (Daejeon, Korea).
cBD Biosciences Clontech (Palo Alto, Calif, USA).

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