Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production

Table 2

List of primers used in PCR experiments.

Primer	Primer sequence^a	Gene to be amplified	Template
Primer 1	aaaaccgttgatatctttgcaaacgggcatgactcctgactttt	aldA promoter	E. coli W3110 chromosome
Primer 2	aaaaccgttgaattcctcctgtgatttatatgtttgttttc
Primer 3	aaaaccgttgatatctgcagaatgaagggtgatttatgtgatttg	udp promoter	E. coli W3110 chromosome
Primer 4	aaaaccgttgaattcctcctctgtgaatcggtttagtcaga
Primer 5	ggaattcatgagtaaaggagaagaacttt	GFP	pGFPuv
Primer 6	cccaagcttttatttgatgagctcatcc

^aRestriction enzyme sites are shown in bold.