Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production

Table 3

Proteins identified from 2DE.


Spot no.	Protein name	Method for identity	Accession no.	p^I/Mw^a (kDa)	Protein description	Fold change^b
Spot no.	Protein name	Method for identity	Accession no.	p^I/Mw^a (kDa)	Protein description	O1/G1	O2/G2

1	AcnB	Gel match	P36683	5.24/75.9	Aconitate hydratase 2
2	AsnS	Gel match	P17242	5.64/92.8	Asparaginyl-tRNA synthetase	—	—
3	AceF	Gel match	P06959	5.01/77.5	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex		—
4	DnaK	Gel match	P04475	4.81/69.6	Chaperone protein DnaK
5	PtsI	Gel match	P08839	4.78/59.8	Phosphoenolpyruvate-protein phosphotransferase		3
6	AldA	MS/MS	P25553	5.07/52.2	Aldehyde dehydrogenase A	Appeared	Appeared
7	MopA (GroEL)	Gel match	P06139	4.85/56.7	60 kDa chaperonin (GroEL protein)
8	Tig	Gel match	P22257	4.83/51.0	Trigger factor (TF)	—	—
9	HtpG	Gel match	P10413	5.06/65.6	Chaperone protein HtpG (Heat shock protein HtpG)	—	—
10	AtpD	Gel match	P00824	4.90/47.7	ATP synthase beta chain	—	—
11	Icd (IcdA)	Gel match	P08200	5.02/46.0	Isocitrate dehydrogenase	—
12	AceA1	MS/MS	P05313	5.19/44.1 5.12/44.1	Isocitrate lyase	5	7
	AceA2	MS/MS		5.01/33.8	Isocitrate lyase fragment	6	7
13	GlnA	Gel match	P06711	5.25/53.8	Glutamine synthetase	—	—
14	IlvC	Gel match	P05793	5.26/52.0	Ketol-acid reductoisomerase	—	—
15	GlpK	Gel match	P08859	5.30/50.6	Glycerol kinase (Glycerokinase)	—	—
16	Eno	Gel match	P08324	5.34/46.5 5.29/46.2	Enolase (2-phosphoglycerate dehydratase)	—
17	TufA (EF-Tu)	Gel match	P02990	5.32/44.6	Elongation factor Tu (EF-Tu)	—	—
18	FabD (TfpA)	Gel match	P25715	5.37/44.8	Malonyl CoA-acyl carrier protein transacylase		—
19	LeuC	Gel match	P30127	5.42/44.4 5.95/51.6	3-isopropylmalate dehydratase large subunit		—
20	FadB	MS/MS	P21177	5.84/79.5	Fatty oxidation complex alpha subunit	Appeared	Appeared
21	SdhA	Gel match	P10444	5.74/63.7	Succinate dehydrogenase flavoprotein subunit	—	—
22	OppA	Gel match	P23843	5.93/56.1	Periplasmic oligopeptide-binding protein		—
23	TrpD	Gel match	P00904	6.08/55.9	Anthranilate synthase component II; Anthranilate	—	—
24	GuaB (GuaR)	Gel match	P06981	6.01/55.0	Inosine--monophosphate dehydrogenase
25	AtpA	Gel match	P00822	5.84/53.1	ATP synthase alpha chain	—
26	DppA	Gel match	P23847	5.69/52.1	Periplasmic dipeptide transport protein	—	—
27	GlyA	Gel match	P00477	6.04/45.9 5.94/46.1	Serine hydroxymethyltransferase (Serine methylase)	—	—
28	CarA (PyrA)	Gel match	P00907	5.91/44.0	Carbamoyl-phosphate synthase small chain	—	—
29	Unknown	MS/MS	—	—	—	Disappeared	—
30	Unknown	MS/MS	—	—	—	Disappeared	Disappeared
31	FadA	MS/MS	P21151	6.31/40.9	Fatty oxidation complex beta subunit	Appeared	Appeared
32	Fba	Gel match	P11604	5.55/40.6	Fructose-bisphosphate aldolase class II	—	—
33	SerC (PdxF)	Gel match	P23721	5.34/40.2	Phosphoserine aminotransferase
34	SucC	Gel match	P07460	5.30/42.3	Succinyl-CoA synthetase beta chain
35	LivJ	MS/MS	P02917	5.28/41.9	Leu/Ile/Val-binding protein	—	—
36	Pgk	Gel match	P11665	5.07/41.9 5.02/41.7	Phosphoglycerate kinase	—
37	MalE	MS/MS	P02928	5.08/41.1	Maltose-binding periplasmic protein	Appeared	Appeared
38	LivK	Gel match	P04816	5.00/41.4	Leucine-specific binding protein	—	—
39	RfaD (HtrM)	Gel match	P17963	4.85/36.8	ADP-L-glycero-D-manno-heptose-6-epimerase	—	—
40	PotD	Gel match	P23861	4.77/35.8	Spermidine/putrescine-binding periplasmic protein	—	—
41	TalB	Gel match	P30148	5.01/35.8	Transaldolase B	—
42	Tsf (EF-Ts)	Gel match	P02997	5.15/33.6	Elongation factor Ts (EF-Ts)	—
43	Mdh	MS/MS	P06994	5.55/35.5	Malate dehydrogenase		—
44	CysK	Gel match	P11096	5.81/36.0	Cysteine synthase A	—	—
45	ManX (PtsL)	Gel match	P08186	5.17/26.1	PTS system, mannose-specific IIAB component		—
46	Unknown	MS/MS	—	—	—	2	3
47	AroG	Gel match	P00886	6.12/39.4	Phospho-2-dehydro-3-deoxyheptonate aldolase	2
48	Sbp	Gel match	P06997	6.49/49.5	Sulfate-binding protein	2
49	GapA	Gel match	P06977	6.58/36.3	Glyceraldehyde 3-phosphate dehydrogenase A	—	—
50	PyrB	Gel match	P00479	6.13/35.3	Aspartate carbamoyltransferase catalytic chain	—	—
51	FkpA	Gel match	P45523	7.08/33.2	FKBP-type peptidyl-prolyl cis-trans isomerase FkpA	—	—
52	SucD	MS/MS	P07459	6.31/29.6	Succinyl-CoA synthetase alpha chain
53	GapA	MS/MS	P06977	6.58/23.0	No. 49 fragment	Disappeared	Disappeared
54	GlnH	MS/MS	P10344	6.87/24.9	Glutamine-binding periplasmic protein	—	—
55	SodA	Gel match	P00448	6.44/22.9	Superoxide dismutase [Mn] (MnSOD)	—	—
56	RbsB	MS/MS	P02925	5.92/29.1	D-ribose-binding periplasmic protein	Appeared	Appeared
57	Udp	Gel match	P12758	5.86/27.9	Uridine phosphorylase (UDRPase)	Appeared	Appeared
58	YadK	Gel match	P37016	5.55/28.4	Protein YadK	—	—
59	TpiA (Tpi)	Gel match	P04790	5.57/26.9	Triosephosphate isomerase	—	—
60	Cdd	MS/MS	P13652	5.08/31.5	Cytidine deaminase	Appeared	Appeared
		MS/MS		5.42/31.5		Appeared	Appeared
61	TrpA	Gel match	P00928	5.30/28.7	Tryptophan synthase alpha chain	—	—
62	SspA (Ssp)	Gel match	P05838	5.24/26.6	Stringent starvation protein A	—	—
63	HisJ	Gel match	P39182	5.05/28.6	Histidine-binding periplasmic protein	—	—
64	FliY	Gel match	P39174	5.01/26.2 5.11/25.8	Cystine-binding periplasmic protein		3
65	HdhA (HsdH)	Gel match	P25529	5.17/25.0	7-alpha-hydroxysteroid dehydrogenase
66	Upp (UraP)	Gel match	P25532	5.29/23.8	Uracil phosphoribosyltransferase	—	—
67	GrpE	Gel match	P09372	4.68/25.5	GrpE protein (HSP-70 cofactor)	—	2
68	AccB (FabE)	Gel match	P02905	4.57/22.0	Biotin carboxyl carrier protein of acetyl-CoA carboxylase	2	2
69	YfdX	MS/MS	P76520	5.38/23.0	Protein yfdX	Disappeared	Disappeared
70	AhpC	Gel match	P26427	5.01/21.5	Alkyl hydroperoxide reductase C22 protein
71	Crr	Gel match	P08837	4.57/20.0 4.68/18.9	PTS system, glucose-specific IIA component	—	—
72	DksA	Gel match	P18274	4.90/18.7	DnaK suppressor protein	—
73	AroK	Gel match	P24167	5.30/17.9	Shikimate kinase I	—	—
74	SodB	Gel match	P09157	5.53/22.1	Superoxide dismutase [Fe]	2
75	PpiB	Gel match	P23869	5.51/17.7	Peptidyl-prolyl cis-trans isomerase B	—
76	RplI	Gel match	P02418	6.20/19.8 6.17/15.7	50S ribosomal protein L9
77	YbdQ	Gel match	P39177	6.08/15.5	Unknown protein from 2D-page	—	—
78	RbfA	Gel match	P09170	6.00/15.6	Ribosome-binding factor A	—	—
79	RplU	Gel match	P02422	6.71/10.3	50S ribosomal protein L21	2	3
80	Hns	Gel match	P08936	5.45/15.6	DNA-binding protein H-NS (Histone-like protein HLP-II)	—
81	Ndk	Gel match	P24233	5.59/15.2	Nucleoside diphosphate kinase (NDP kinase)	—	—
82	AtpC	Gel match	P00832	5.48/14.8	ATP synthase epsilon chain	—	—
83	RpsF	Gel match	P02358	5.31/15.8 5.15/15.8 5.26/15.8	30S ribosomal protein S6	—	—
84	Bcp	Gel match	P23480	5.02/15.8	Bacterioferritin comigratory protein		2
85	GreA	Gel match	P21346	4.68/15.9	Transcription elongation factor GreA	2	Disappeared
86	GroES (MopB)	Gel match	P05380	5.15/15.6	10 kDa chaperonin (GroES protein)	—	—
87	YfiD	MS/MS	P33633	5.09/14.3	Protein YfiD	—	—
88	UspA	Gel match	P28242	5.14/15.1	Universal stress protein A	—	—
89	YjgF	Gel match	P39330	5.29/13.0	Protein YjgF
90	TrxA (TsnC)	Gel match	P00274	4.67/11.5	Thioredoxin 1	—	—
91	HdeB	Gel match	P26605	4.85/11.2	Protein HdeB (10K-L protein)	—	3
92	YccU	MS/MS	P75874	6.72/14.7	Protein YccU; Predicted CoA-binding protein	Appeared	Appeared

^aUnit of the molecular weight (MW) is kDa.
^bFold change: 0 ~0.3-fold, 3 ; 0.3 ~0.5-fold, 2 ; 0.5 ~0.6-fold, ; 1.5-fold, ; 2-fold, 2 ; fold change, fold number.

BioMed Research International

Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production

Table 3