BioMed Research International / 2008 / Article / Tab 3

Research Article

Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production

Table 3

Proteins identified from 2DE.

Spot no.Protein nameMethod for identityAccession no.pI/Mwa (kDa)Protein descriptionFold changeb
O1/G1O2/G2

1AcnBGel matchP366835.24/75.9Aconitate hydratase 2
2AsnSGel matchP172425.64/92.8Asparaginyl-tRNA synthetase
3AceFGel matchP069595.01/77.5Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
4DnaKGel matchP044754.81/69.6Chaperone protein DnaK
5PtsIGel matchP088394.78/59.8Phosphoenolpyruvate-protein phosphotransferase 3
6AldAMS/MSP255535.07/52.2Aldehyde dehydrogenase AAppearedAppeared
7MopA (GroEL)Gel matchP061394.85/56.760 kDa chaperonin (GroEL protein)
8TigGel matchP222574.83/51.0Trigger factor (TF)
9HtpGGel matchP104135.06/65.6Chaperone protein HtpG (Heat shock protein HtpG)
10AtpDGel matchP008244.90/47.7ATP synthase beta chain
11Icd (IcdA)Gel matchP082005.02/46.0Isocitrate dehydrogenase
12AceA1MS/MSP053135.19/44.1 5.12/44.1Isocitrate lyase5 7
AceA2MS/MS5.01/33.8Isocitrate lyase fragment6 7
13GlnAGel matchP067115.25/53.8Glutamine synthetase
14IlvCGel matchP057935.26/52.0Ketol-acid reductoisomerase
15GlpKGel matchP088595.30/50.6Glycerol kinase (Glycerokinase)
16EnoGel matchP083245.34/46.5 5.29/46.2Enolase (2-phosphoglycerate dehydratase)
17TufA (EF-Tu)Gel matchP029905.32/44.6Elongation factor Tu (EF-Tu)
18FabD (TfpA)Gel matchP257155.37/44.8Malonyl CoA-acyl carrier protein transacylase
19LeuCGel matchP301275.42/44.4 5.95/51.63-isopropylmalate dehydratase large subunit
20FadBMS/MSP211775.84/79.5Fatty oxidation complex alpha subunitAppearedAppeared
21SdhAGel matchP104445.74/63.7Succinate dehydrogenase flavoprotein subunit
22OppAGel matchP238435.93/56.1Periplasmic oligopeptide-binding protein
23TrpDGel matchP009046.08/55.9Anthranilate synthase component II; Anthranilate
24GuaB (GuaR)Gel matchP069816.01/55.0Inosine- -monophosphate dehydrogenase
25AtpAGel matchP008225.84/53.1ATP synthase alpha chain
26DppAGel matchP238475.69/52.1Periplasmic dipeptide transport protein
27GlyAGel matchP004776.04/45.9 5.94/46.1Serine hydroxymethyltransferase (Serine methylase)
28CarA (PyrA)Gel matchP009075.91/44.0Carbamoyl-phosphate synthase small chain
29UnknownMS/MSDisappeared
30UnknownMS/MSDisappearedDisappeared
31FadAMS/MSP211516.31/40.9Fatty oxidation complex beta subunitAppearedAppeared
32FbaGel matchP116045.55/40.6Fructose-bisphosphate aldolase class II
33SerC (PdxF)Gel matchP237215.34/40.2Phosphoserine aminotransferase
34SucCGel matchP074605.30/42.3Succinyl-CoA synthetase beta chain
35LivJMS/MSP029175.28/41.9Leu/Ile/Val-binding protein
36PgkGel matchP116655.07/41.9 5.02/41.7Phosphoglycerate kinase
37MalEMS/MSP029285.08/41.1Maltose-binding periplasmic proteinAppearedAppeared
38LivKGel matchP048165.00/41.4Leucine-specific binding protein
39RfaD (HtrM)Gel matchP179634.85/36.8ADP-L-glycero-D-manno-heptose-6-epimerase
40PotDGel matchP238614.77/35.8Spermidine/putrescine-binding periplasmic protein
41TalBGel matchP301485.01/35.8Transaldolase B
42Tsf (EF-Ts)Gel matchP029975.15/33.6Elongation factor Ts (EF-Ts)
43MdhMS/MSP069945.55/35.5Malate dehydrogenase
44CysKGel matchP110965.81/36.0Cysteine synthase A
45ManX (PtsL)Gel matchP081865.17/26.1PTS system, mannose-specific IIAB component
46UnknownMS/MS2 3
47AroGGel matchP008866.12/39.4Phospho-2-dehydro-3-deoxyheptonate aldolase2
48SbpGel matchP069976.49/49.5Sulfate-binding protein2
49GapAGel matchP069776.58/36.3Glyceraldehyde 3-phosphate dehydrogenase A
50PyrBGel matchP004796.13/35.3Aspartate carbamoyltransferase catalytic chain
51FkpAGel matchP455237.08/33.2FKBP-type peptidyl-prolyl cis-trans isomerase FkpA
52SucDMS/MSP074596.31/29.6Succinyl-CoA synthetase alpha chain
53GapAMS/MSP069776.58/23.0No. 49 fragmentDisappearedDisappeared
54GlnHMS/MSP103446.87/24.9Glutamine-binding periplasmic protein
55SodAGel matchP004486.44/22.9Superoxide dismutase [Mn] (MnSOD)
56RbsBMS/MSP029255.92/29.1D-ribose-binding periplasmic proteinAppearedAppeared
57UdpGel matchP127585.86/27.9Uridine phosphorylase (UDRPase)AppearedAppeared
58YadKGel matchP370165.55/28.4Protein YadK
59TpiA (Tpi)Gel matchP047905.57/26.9Triosephosphate isomerase
60CddMS/MSP136525.08/31.5Cytidine deaminaseAppearedAppeared
MS/MS5.42/31.5AppearedAppeared
61TrpAGel matchP009285.30/28.7Tryptophan synthase alpha chain
62SspA (Ssp)Gel matchP058385.24/26.6Stringent starvation protein A
63HisJGel matchP391825.05/28.6Histidine-binding periplasmic protein
64FliYGel matchP391745.01/26.2 5.11/25.8Cystine-binding periplasmic protein 3
65HdhA (HsdH)Gel matchP255295.17/25.07-alpha-hydroxysteroid dehydrogenase
66Upp (UraP)Gel matchP255325.29/23.8Uracil phosphoribosyltransferase
67GrpEGel matchP093724.68/25.5GrpE protein (HSP-70 cofactor)2
68AccB (FabE)Gel matchP029054.57/22.0Biotin carboxyl carrier protein of acetyl-CoA carboxylase2 2
69YfdXMS/MSP765205.38/23.0Protein yfdXDisappearedDisappeared
70AhpCGel matchP264275.01/21.5Alkyl hydroperoxide reductase C22 protein
71CrrGel matchP088374.57/20.0 4.68/18.9PTS system, glucose-specific IIA component
72DksAGel matchP182744.90/18.7DnaK suppressor protein
73AroKGel matchP241675.30/17.9Shikimate kinase I
74SodBGel matchP091575.53/22.1Superoxide dismutase [Fe]2
75PpiBGel matchP238695.51/17.7Peptidyl-prolyl cis-trans isomerase B
76RplIGel matchP024186.20/19.8 6.17/15.750S ribosomal protein L9
77YbdQGel matchP391776.08/15.5Unknown protein from 2D-page
78RbfAGel matchP091706.00/15.6Ribosome-binding factor A
79RplUGel matchP024226.71/10.350S ribosomal protein L212 3
80HnsGel matchP089365.45/15.6DNA-binding protein H-NS (Histone-like protein HLP-II)
81NdkGel matchP242335.59/15.2Nucleoside diphosphate kinase (NDP kinase)
82AtpCGel matchP008325.48/14.8ATP synthase epsilon chain
83RpsFGel matchP023585.31/15.8 5.15/15.8 5.26/15.830S ribosomal protein S6
84BcpGel matchP234805.02/15.8Bacterioferritin comigratory protein 2
85GreAGel matchP213464.68/15.9Transcription elongation factor GreA2 Disappeared
86GroES (MopB)Gel matchP053805.15/15.610 kDa chaperonin (GroES protein)
87YfiDMS/MSP336335.09/14.3Protein YfiD
88UspAGel matchP282425.14/15.1Universal stress protein A
89YjgFGel matchP393305.29/13.0Protein YjgF
90TrxA (TsnC)Gel matchP002744.67/11.5Thioredoxin 1
91HdeBGel matchP266054.85/11.2Protein HdeB (10K-L protein)3
92YccUMS/MSP758746.72/14.7Protein YccU; Predicted CoA-binding proteinAppearedAppeared

aUnit of the molecular weight (MW) is kDa.
bFold change: 0 ~0.3-fold, 3 ; 0.3 ~0.5-fold, 2 ; 0.5 ~0.6-fold, ; 1.5-fold, ; 2-fold, 2 ; fold change, fold number .

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