Research Article | Open Access
O. Carvajal-Zarrabal, P. M. Hayward-Jones, Z. Orta-Flores, C. Nolasco-Hipólito, D. M. Barradas-Dermitz, M. G. Aguilar-Uscanga, M. F. Pedroza-Hernández, "Effect of Hibiscus sabdariffa L. Dried Calyx Ethanol Extract on Fat Absorption-Excretion, and Body Weight Implication in Rats", BioMed Research International, vol. 2009, Article ID 394592, 5 pages, 2009. https://doi.org/10.1155/2009/394592
Effect of Hibiscus sabdariffa L. Dried Calyx Ethanol Extract on Fat Absorption-Excretion, and Body Weight Implication in Rats
The effect of Hibiscus sabdariffa L. (Hs) calyx extract on fat absorption-excretion and body weight in rats, was investigated. Rats were fed with either a basal diet (SDC = Control diet) or the same diet supplemented with Hs extracts at 5%, 10% and 15% ( , and ). Only did not show significant increases in weight, food consumption and efficiency compared to . The opposite occurred in group which showed a significant decrease for these three parameters. The responses were similar to , with the exception of food consumption. In both and groups, no body weight loss was observed; however, only in the latter group was there a significantly greater amount of fatty acids found in feces. A collateral effect emerging from the study is that components of Hs extract at the intermediate and greater concentrations used in this experiment could be considered possible antiobesity agents.
The scientific groups carrying out studies on Hibiscus sabdariffa L. family Malvaceae calyx ethanol extract in order to ascertain itsphysiological activity-structure relationship are generally located in areas where it isused in food applications and traditional medicine . Advances in thesestudies can be divided into three lines: therapeutic effects on lipid metabolism [2–8]; antihypertensive effects [9–13]; apoptotic effects in gastric carcinoma cells [14, 15]. In several of the studies, [2, 4, 6, 8–11, 13] no extract standardization is reported, although a tendency is observed to overcome this deficiency. Besides the calyx, H. sabdariffa L. leaf has also been subjected to this type of scientific studies, particularly leaf ethanolic extracts, which have been found to influence lipid metabolism [16–20].
Hs calyx extract contains hibiscus acid, or (+)-hydroxycitric acid, known as (+)-HCA [21–23]. Its isomer, -hydroxycitric acid or -HCA, the active ingredient principle present in Garcinia indica and Garcinia cambogia fruits, is an inhibitor of citrate lyase [24, 25], and because of this, it has been proposed as an anti-obesity agent [25–30]. Tee et al.  reported that hydroxycitric acid (no isomer specified), present in Hs calyx extract, inhibits fat production from carbohydrates in experiments carried out on rats. In addition, Carvajal-Zarrabal et al.  suggested that racemization of (+)-HCA to -HCA by the intestinal flora may be a possible explanation to warrant the significant decrease in triacylglycerols in the experiment carried out on rats supplemented with Hs extract. This proposal agrees with the generalization made by Borriello et al. , which establishes that phytochemicals absorbed in the intestine can be transformed by colonic bacteria, resulting in serum components different from the original phytochemicals.
Therapeutic effects reported in studies carried out on animal models supplemented with Roselle, Hibiscus sabdariffa L. extracts include its influence on lipid metabolism as well as its antihypertensive and apoptotic actions. The aim of the present study was to research the action of Hs calyx extract on fat absorption, excretion, and body weight, as no information was encountered referring specifically to these aspects of lipid metabolism.
2. Materials and Methods
2.1. Plant Material and Extract Preparation
The air dried calyces along with a sample of the flowering plant of Hibiscus sabdariffa were acquired from the local market in Veracruz, Mexico. The sample was authenticated as Hibiscus sabdariffa L. by Prof. Sergio Avendaño and registered as O.Carvajal 001 at the Herbarium of the Ecology Institute A.C., Xalapa, Veracruz. Mexico. The calyces (135 g) were placed in a flask and 500 mL (96%) ethanol were added. The content was left for 8 days, with an occasional shaking to increase the extraction capacity. The macerated substance was filtered and concentrated in a rotary evaporator at . The solid mass obtained from the evaporated extract was approximately 7.5% w/v. The concentrated plant extract was stored at until used. Its viscosity at was 1200 c.p.
2.2. Animals and Diets
The experimental protocol for animal experiments was approved by the Animal Ethic Committee, Chemical-Biology Area, University of Veracruz (Program: Hibiscus sabdariffa Part I 2004–2008). Forty Male Sprague-Dawley rats (6 weeks old, 250 to 350 g in weight) were purchased from Harlan Teklad, Co. (Mexico City) and individually housed in stainless steel mesh cages in a temperature-controlled room ( regulated by an electronic timer) with a 12-hours light/dark cycle. They had free access to food and non-ionized water throughout the feeding period. The basal diet was prepared according to the American Institute of Nutrition  and is shown in Table 1. Lard (10 g/100 g diet) was employed as the source of dietary fat, and cholesterol and cholic acid were added at 1 and 0.25 g/100 g diet, respectively. The experimental diet is the basal diet plus ethanol dried extract of Hibiscus sabdariffa calyces at levels of 5, 10, and 15 g of extract/100 g diet. Animals were fed the basal diet for one week in order to develop an atherogenic condition (cholesterol ≥ 220 mg/dL, atherogenic index defined as total cholesterol, cHDL/cHDL ≥ 2.5). Thereafter, they were divided into four groups (10 rats each). The control group was maintained on the basal diet and three groups of rats, designated as SD5, SD10, SD15, received the respective experimental diet for 4 weeks. The parameters that were quantified are directly related to the digestive process and its effects; as this lasts approximately 3 hours, its effect on body weight can be measured with confidence and reproducibility within an experimental period of 4 weeks. Diets were prepared once a week and stored in powdered form at until feeding. Body weight and food intake were measured daily. Feces were collected during the last 5 days and freeze-dried. At the end of the experimental period, diets were withdrawn for at least four hours.
2.3. Determination of Fat in Feces
Fecal fats were extracted according to the method of Jeejeebhoy et al.. Briefly, 1 g freeze-dried feces was acidified with 2 dropsconcentrated HCl to release free fatty acids, and sequentially extracted with solvent No.1 (heptane: diethyl ether: 95% ethanol = 1 : 1 : 1) and solvent No.2 (heptane: diethyl ether: 95% ethanol: water=1 : 1 : 1 : 1). The lipid extract was vacuum dried (Gallenkamp Oven Mod. UAF- 570-0300) and weighed. The apparent absorption rate (%) of dietary fat was calculated as 100 x [amount of daily fatty acid intake – amount of fecal fatty acids excreted]/[amount of daily fatty acid intake]. To identify the individual fatty acids excreted into the feces, the lipid extract was saponified with an ethanol-KOH solution, and the saponified fatty acids were acidified with an solution, diluted with an equal volume of , and methylated with an / solution (1 : 115) as described by Ikeda et al. . Fatty acids were determined by gas chromatography (Hewlett Packard 5890, Palo Alto, CA.) with pentadecanoic acid as an internal standard. All chemicals used were analytical grade.
2.4. Statistical Analysis
The obtained data were expressed as mean standard deviation of means (). A one-way analysis of variance (ANOVA) was used to compare the means of the studied groups with post hoc Duncan multiple range tests at 5% and 1% for those results where a significant difference was indicated. Minitab version 12 statistical software was used.
3.1. Atherogenic Condition, Growth, and Diet Consumption Parameters
Animals fed the basal diet attained an atherogenic condition, their levels of cholesterol and their atherogenic index being mg/dL and 6.1, respectively. Table 2 shows growth, food consumption, and weight parameters for both the control group and the three experimental groups supplemented with Hs extract (SD5, SD10, and SD15). No significant differences were observed between the experimental groups and control as regards body weight; however, body weight gain in SD10 and SD15 groups was significantly less than in control group . Food consumption in the experimental groups (SD5, SD10, SD15) decreased with Hs extract dose, but this only became significant in the SD15 group. Food efficiency for the SD5 group was the same as for control ; however, for SD10 and SD15 groups, a significant decrease in this parameter was observed ( and , resp.) compared to control. Feces weight (g/d) in all experimental groups varied significantly compared to the control, with a significant increase observed in the SD5 treated group and a significant decrease in the SD10 and SD15 treated groups.
|Statistical differences , when compared with the control group.|
3.2. Apparent Fecal Fat Absorption and Excretion
The results shown in Table 3 reflect the effect of the diet the animals were subjected to in this study, concerning fat absorption and excretion. In the case of SD5, fat absorption was significantly lower compared to control group . A tendency toward higher excretion was observed in the fecal fatty acid profiles of experimental groups, as compared to control. Specifically, SD5 group showed an increase for all fatty acids in feces, significantly so for 16:0 (palmitic), 18 : 0 (stearic), 18 : 1 (oleic), and 18 : 2 (n-6) (linoleic). Fatty acids measured in groups SD10 and SD15 showed an increase in four cases: 16 : 0 (palmitic), 16 : 1 (palmitoleic), 18 : 1 (oleic), and 18 : 2 (n-6) (linoleic); only for palmitoleic was the increase not significant.
|Statistical differences (, when compared with the control group.|
Both experimental (SD5, SD10, and SD15) and control groups were fed ad libitum. Only SD5 group did not show a significant difference in the three parameters studied: weight gain, food consumption, and efficiency, as compared to control. These results were different between groups SD5 and SD15, SD5 showing behavior similar to control, while in SD15 a significant decrease in all three parameters was observed. SD10 was similar to SD15, except in the case of food consumption. Considering food efficiency comprises both weight gain and food consumption, and that, as a result, dietary components and their effect on body weight are related; it is evident that Hs extracts, at intermediate and greater concentrations used in these experiments (SD10 and SD15), by not increasing body weight, reveal themselves as potential antiobesity agents.
On the other hand, the SD5 group absorbed the least amount of fat, exhibited an increase in all fatty acids in feces resulting from fat hydrolysis, and did not lose weight, the latter behavior being similar to control. Fatty acid excretion in , however, was less than in SD5 group. One possible explanation for this behavior in control and SD5 groups, where weight gain was similar though with differential lipid excretion, could be due to weight gain in SD5 group basically through carbohydrate absorption; this assumes that Hs extract components at this level of concentration do not exert an inhibitory effect on pancreatic amylase.
Lower weight gain in SD10 and SD15 groups, added to their similar total lipid absorption, though different in excreted fatty acid type (greater palmitic, oleic, and linoleic and lower stearic acids as compared to control) seems to indicate that at these concentrations Hs extract components could inhibit pancreatic amylase, as reported by Hansawasdi et al. [36, 37], who identified Hibiscus acid, or (+)-HCA, as responsible for this action. This would consequently prevent polysaccharide unfolding and absorption. Mention should be made of the significant decrease in food consumption observed in the SD15 group, possibly related to a dietary palatability problem when Hs extract concentration was increased. Significantly increased C16:0 excretion, observed in all groups, is attributable to Hs extract chemical components. It is relevant to recall that the fat administered in this experiment was lard, rich in triacylglicerols with C16:0 in sn-2 position. Renaud et al.  observed that C16:0 interesterification in fat triacylglycerols results in a greater fat secretion in experimental animal feces accompanied by a decrease in TAG and cholesterol levels, including HDL cholesterol. We consider that this result is due to, amongst other factors, the specificity of lingual and gastric lipases which hydrolyse esters in positions 1 and 3, the latter being twice as susceptible as the former. In order to explain the effect of Hs extract chemical components, in their original state or modified by the intestinal flora, on increased C16:0 concentration (20, 7, and 4.5 times in SD5, SD10, and SD15, respectively, compared to ), as observed in the present study of fecal fat, the following hypotheses are proposed: inactivation of lipases; impediment of 2-monoacylglycerol uptake into the enterocyte, due to a competitive saturation of the specific transport system , or interesterification of the C16:0 from sn-2 to sn-1 or sn-3. Validation of these hypotheses requires further study.
It can be concluded that animals kept on a diet supplemented with Hs calyx ethanol extract showed significant C16:0 excretion in feces. The three hypotheses proposed to explain this excretion need subsequent testing and validation. Besides, a collateral effect emerging from the study is that Hs extract components at the intermediate and greater concentrations used in this experiment could be considered possible anti-obesity agents, through their tendency to inhibit α-amylase.
The author would like to thank Ma. Remedios Mendoza-López, M. Sc., and to J. Samuel Cruz-Sánchez, Ph.D., from the University of Veracruz (Support Services in Analytical Resolution, SARA), for their support in carrying out GC measurements of fecal samples.
- I. A. Ross, Medicinal Plants of the World Vol I: Chemical Constituents, Traditional and Modern Uses, Humana Press, Totawa, NJ, USA, 2nd edition, 2003.
- S. S. El-Saadany, M. Z. Sitohy, S. M. Labib, and R. A. El-Massry, “Biochemical dynamics and hypocholesterolemic action of Hibiscus sabdariffa (Karkade),” Die Nahrung, vol. 35, no. 6, pp. 567–576, 1991.
- C.-J. Wang, J.-M. Wang, W.-L. Lin, C.-Y. Chu, F.-P. Chou, and T.-H. Tseng, “Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats,” Food and Chemical Toxicology, vol. 38, no. 5, pp. 411–416, 2000.
- C. J. Wang, U.S. patent no. 6,849,278 B2, February 2005.
- C.-C. Chen, J.-D. Hsu, S.-F. Wang et al., “Hibiscus sabdariffa extract inhibits the development of atherosclerosis in cholesterol-fed rabbits,” Journal of Agricultural and Food Chemistry, vol. 51, no. 18, pp. 5472–5477, 2003.
- O. Carvajal-Zarrabal, S. M. Waliszewski, D. M. Barradas-Dermitz et al., “The consumption of Hibiscus sabdariffa dried calyx ethanolic extract reduced lipid profile in rats,” Plant Foods for Human Nutrition, vol. 60, no. 4, pp. 153–159, 2005.
- V. Hirunpanich, A. Utaipat, N. P. Morales et al., “Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L. in hypercholesterolemic rats,” Journal of Ethnopharmacology, vol. 103, no. 2, pp. 252–260, 2006.
- T.-L. Lin, H.-H. Lin, C.-C. Chen, M.-C. Lin, M.-C. Chou, and C.-J. Wang, “Hibiscus sabdariffa extract reduces serum cholesterol in men and women,” Nutrition Research, vol. 27, no. 3, pp. 140–145, 2007.
- M. Haji Faraji and A. H. Haji Tarkhani, “The effect of sour tea (Hibiscus sabdariffa) on essential hypertension,” Journal of Ethnopharmacology, vol. 65, no. 3, pp. 231–236, 1999.
- P. C. Onyenekwe, E. O. Ajani, D. A. Ameh, and K. S. Gamaniel, “Antihypertensive effect of roselle (Hibiscus sabdariffa) calyx infusion in spontaneously hypertensive rats and a comparison of its toxicity with that in wistar rats,” Cell Biochemistry and Function, vol. 17, no. 3, pp. 199–206, 1999.
- I. P. Odigie, R. R. Ettarh, and S. A. Adigun, “Chronic administration of aqueous extract of Hibiscus sabdariffa attenuates hypertension and reverses cardiac hypertrophy in 2K-1C hypertensive rats,” Journal of Ethnopharmacology, vol. 86, no. 2-3, pp. 181–185, 2003.
- A. Herrera-Arellano, S. Flores-Romero, M. A. Chávez-Soto, and J. Tortoriello, “Effectiveness and tolerability of a standardized extract from Hibiscus sabdariffa in patients with mild to moderate hypertension: a controlled and randomized clinical trial,” Phytomedicine, vol. 11, no. 5, pp. 375–382, 2004.
- M. Ajay, H. J. Chai, A. M. Mustafa, A. H. Gilani, and M. R. Mustafa, “Mechanisms of the anti-hypertensive effect of Hibiscus sabdariffa L. calyces,” Journal of Ethnopharmacology, vol. 109, no. 3, pp. 388–393, 2007.
- Y.-C. Chang, K.-X. Huang, A.-C. Huang, Y.-C. Ho, and C.-J. Wang, “Hibiscus anthocyanins-rich extract inhibited LDL oxidation and oxLDL-mediated macrophages apoptosis,” Food and Chemical Toxicology, vol. 44, no. 7, pp. 1015–1023, 2006.
- H.-H. Lin, J.-H. Chen, W.-H. Kuo, and C.-J. Wang, “Chemopreventive properties of Hibiscus sabdariffa L. on human gastric carcinoma cells through apoptosis induction and JNK/p38 MAPK signaling activation,” Chemico-Biological Interactions, vol. 165, no. 1, pp. 59–75, 2007.
- M. M. Essa, P. Subramanian, G. Suthakar et al., “Influence of Hibiscus sabdariffa (Gongura) on the levels of circulatory lipid peroxidation products and liver marker enzymes in experimental hyperammonemia,” Journal of Applied Biomedicine, vol. 4, no. 1, pp. 53–58, 2006.
- M. M. Essa, P. Subramanian, T. Manivasagam, K. B. Dakshayani, S. Subash, and R. Sivaperumal, “Protective influence of Hibiscus sabdariffa, an edible medicinal plant, on tissue lipid peroxidation and antioxidant status in hyperammonemic rats,” African Journal of Traditional, Complementary and Alternative Medicines, vol. 3, no. 3, pp. 10–21, 2006.
- M. M. Essa and P. Subramanian, “Effect of Hibiscus sabdariffa on lipid peroxidation in hyperammonemic rats,” Journal Cell Tissue Research, vol. 6, pp. 819–824, 2006.
- M. M. Essa and P. Subramanian, “Hibiscus sabdariffa affects ammonium chloride-induced hyperammonemic rats,” Evidence-Based Complementary and Alternative Medicine, vol. 4, no. 3, pp. 321–325, 2007.
- M. M. Essa and P. Subramanian, “Influence of Hibiscus sabdariffa on the rhythmic alterations of liver markers in experimental hyperammonemic rats,” Biological Rhythm Research, vol. 40, no. 3, pp. 273–278, 2009.
- I. Ibnusaud and G. Thomas, “Biologically interesting chiral 3,4-disubstituted pyrrolidines from optically active hydroxycitric acid lactones,” Tetrahedron Letters, vol. 44, no. 6, pp. 1247–1249, 2003.
- B. S. Jena, G. K. Jayaprakasha, R. P. Singh, and K. K. Sakariah, “Chemistry and biochemistry of (-)-hydroxycitric acid from Garcinia,” Journal of Agricultural and Food Chemistry, vol. 50, no. 1, pp. 10–22, 2002.
- T. Yamada, H. Hida, and Y. Yamada, “Chemistry, physiological properties and microbial production of hydrocytric acid,” Applied Microbiology and Biotechnology, vol. 75, pp. 977–982, 2007.
- A. C. Sullivan, J. G. Hamilton, O. N. Miller, and V. R. Wheatley, “Inhibition of lipogenesis in rat liver by (-)-hydroxycitrate,” Archives of Biochemistry and Biophysics, vol. 150, no. 1, pp. 183–190, 1972.
- A. C. Sullivan, J. Triscari, and J. G. Hamilton, “Effect of (-) hydroxycitrate upon the accumulation of lipid in the rat: I. Lipogenesis,” Lipids, vol. 9, no. 2, pp. 121–128, 1974.
- S. B. Heymsfield, D. B. Allison, J. R. Vasselli, A. Pietrobelli, D. Greenfield, and C. Nuñez, “Garcinia cambogia (hydroxycitric acid) as a potential antiobesity agent: arandomized controlled trial,” Journal of the American Medical Association, vol. 280, no. 18, pp. 1596–1600, 1998.
- K. Hayamizu, Y. Ishii, I. Kaneko et al., “Effects of long-term administration of Garcinia cambogia extract on visceral fat accumulation in humans: a placebo-controlled double blind trial,” Journal of Oleo Science, vol. 50, pp. 805–812, 2001.
- K. Hayamizu, T. Ishii, I. Kaneko et al., “No-Observed-Adverse-Effect Level (NOAEL) and sequential-high-doses administration study on Garcinia cambogia extract in humans,” Journal of Oleo Science, vol. 51, no. 4, pp. 365–369, 2002.
- K. Hayamizu, Y. Ishii, I. Kaneko et al., “Effect of Garcinia cambogia (hydroxicitric acid) on visceral fat accumulation: a double-blind, randomized, placebo-controlled trial,” Current Therapeutic Research, vol. 64, pp. 551–567, 2003.
- K. Hayamizu, Y. Ishii, N. Shigematsu et al., “Safety of Garcinia cambogia extract in healthy men: high-doses administration study,” Journal of Oleo Science, vol. 52, pp. 499–504, 2003.
- P. L. Tee, S. Yusof, S. Mohamed, and N. A. Umar, “Mustapha, effect of roselle (Hibiscus sabdariffa L.) on serum lipid of sprague dawley rats,” Nutrition and Food Science, vol. 32, pp. 190–196, 2002.
- S. P. Borriello, K. D. R. Setchell, M. Axelson, and A. M. Lawson, “Production and metabolism of lignans by the human faecal flora,” Journal of Applied Bacteriology, vol. 58, no. 1, pp. 37–43, 1985.
- P. G. Reeves, F. H. Nielsen, and G. C. Fahey Jr., “AIN-93 purified diets for laboratory rodents: final report of the american institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet,” Journal of Nutrition, vol. 123, no. 11, pp. 1939–1951, 1993.
- K. N. Jeejeebhoy, S. Ahmad, and G. Kozak, “Determination of fecal fats containing both medium and long chain triglycerides and fatty acids,” Clinical Biochemistry, vol. 3, no. 1, pp. 157–163, 1970.
- I. Ikeda, H. Yoshida, M. Tomooka et al., “Effects of long-term feeding of marine oils with different positional distribution of eicosapentaenoic and docosahexaenoic acids on lipid metabolism, eicosanoid production, and platelet aggregation in hypercholesterolemic rats,” Lipids, vol. 33, no. 9, pp. 897–904, 1998.
- C. Hansawasdi, J. Kawabata, and T. Kasai, “-amylase inhibitors from roselle (Hibiscus sabdariffa Linn.) tea,” Bioscience, Biotechnology and Biochemistry, vol. 64, no. 5, pp. 1041–1043, 2000.
- C. Hansawasdi, J. Kawabata, and T. Kasai, “Hibiscus acid as an inhibitor of starch digestion in the Caco-2 cell model system,” Bioscience, Biotechnology and Biochemistry, vol. 65, no. 9, pp. 2087–2089, 2001.
- S. C. Renaud, J. C. Ruf, and D. Petithory, “The positional distribution of fatty acids in palm oil and lard influences their biologic effects in rats,” Journal of Nutrition, vol. 125, no. 2, pp. 229–237, 1995.
- K. Murota and J. Storch, “Uptake of micellar long-chain fatty acid and sn-2-monoacylglycerol into human intestinal Caco-2 cells exhibits characteristics of protein-mediated transport,” Journal of Nutrition, vol. 135, no. 7, pp. 1626–1630, 2005.
Copyright © 2009 O. Carvajal-Zarrabal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.