Immunohistochemical and Proteomic Evaluation of Nuclear Ubiquitous Casein and Cyclin-Dependent Kinases Substrate in Invasive Ductal Carcinoma of the Breast
Figure 1
NUCKS is highly overexpressed in breast cancer. Lanes 2–11: Protein extracts from five pairs of tissue, each pair matching the individual patient, of normal breast (N) and invasive ductal carcinoma GII (Ca) were separated by SDS-PAGE and stained with Coomassie. Lanes 1, 12, and 13: molecular weight standard and proteins extracts of MCF7 cells and an ovarian tumor, respectively. Asterisks indicate the position of NUCKS band. Mr: relative molecular weight. The band of linker histone H1.4 (arrow), a highly abundant housekeeping protein, serves a “gel loading” control. Its intensity is similar in lanes of normal breast, IDC, and MCF7 cells lysates. Identity of the H1.4 band was confirmed by mass spectrometry (Figure 1).
