Generation and Characterization of Novel Human IRAS Monoclonal Antibodies
Analysis of immunoprecipitates revealed that the IRAS mAbs specifically recognized the native state of the IRAS protein. The HEK293 cells were transiently transfected with the empty vector PCMV-myc (negative control) and PCMV-myc-IRAS. Whole cell lysates were prepared and immunoprecipitated with the c-myc mAb or the mAbs DA041, DD015, BE073, BA022, and AH021. Immunoprecipitation of the same lysates using mouse normal IgG did not result in the detection of any protein species. Immunocomplexes were analyzed by western blot with indicated antibodies.
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