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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 985140, 9 pages
Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector
1Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan
2Department of Organ Regeneration, Graduate School of Medicine, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
3Department of Developmental Biology, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki, 305-0901, Japan
4Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, 1-21-20 Korimoto, Kagoshima 890-0065, Japan
Received 12 September 2008; Accepted 13 January 2009
Academic Editor: Vladimir Larionov
Copyright © 2009 Masato Ohtsuka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red fluorescence. Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused switching from red to green fluorescence. Similarly, cotransfection with pCETRIZ and pCAG/NCre resulted in change of green to red fluorescence. These noninvasive systems will be useful in cell lineage analysis, since descendants of cells exhibiting newly activated gene expression can be continuously monitored in noninvasive fashion.
The bacteriophage P1 Cre recombinase, a 38-kDa protein, recognizes specific 34-bp sequences called loxP sites and catalyzes site-specific recombination between two loxP sites [1, 2]. The Cre-loxP system has been considered an important tool for manipulation of genomic sequences and gene expression including tissue-specific activation or inactivation of genes as well as cell-type-specific gene targeting . Araki et al.  first utilized this system for manipulation of mouse preimplantation embryos. They constructed a CAG-CAT-Z transgene, in which expression of the floxed chloramphenicol acetyltransferase (CAT) gene occurs under control of the ubiquitously active cytomegalovirus enhancer/chicken -actin promoter (CAG) . Expression of the lacZ gene coding for bacterial -galactosidase protein, which is located downstream of the floxed CAT sequence in the CAG-CAT-Z transgene, does not occur in the absence of Cre recombinase protein. When Cre-expression vector DNA was injected into the pronuclei of fertilized eggs carrying the CAG-CAT-Z transgene, expression of lacZ protein was induced, indicating that expression of the lacZ gene is induced after removal of the floxed CAT sequence by Cre recombinase. Once such recombination occurs, expression of the lacZ gene in descendant cells will continue throughout embryogenesis.
Using a strategy similar to that of Araki et al. , we previously produced a double-reporter mouse line carrying a pCETZ-17 transgene, which consists of CAG, floxed DNA sequence (containing enhanced green fluorescent protein (EGFP) cDNA), and the lacZ gene . This Cre-loxP-based system was useful for cell lineage analysis in mice. For example, when one blastomere of the 2-cell embryos derived from CETZ-17 transgenic mice was microinjected with Cre-expression vector (pCAG/NCre-5) , its descendant exhibited Cre-mediated recombination in the integrated transgenes and also exhibited lacZ activity. However, the offspring of the other blastomere, into which no injection was performed, remained silent for lacZ activity. In this case, histochemical detection of lacZ activity is always associated with fixation of tissues and subsequent staining with X-Gal, a substrate for lacZ, or by immunodetection using anti--galactosidase antibodies. This procedure often hampers serial microscopic observation of cells expressing newly activated gene products, and some tissues (i.e., adult ovaries, oviducts, and epididymides) are rich in endogenous activity for lacZ (unpublished data).
In this study, we constructed two new plasmids, pCRTEIL and pCETRIZ, as double-reporter transgenes. pCRTEIL contains CAG, floxed sequence (HcRed1 cDNA and CAT gene), EGFP cDNA, internal ribosomal entry site (IRES) from the encephalomyocarditis virus, and firefly luciferase (luc) cDNA. pCETRIZ contains CAG, floxed EGFP cDNA and CAT gene, HcRed1 cDNA, IRES, and lacZ gene. For example, cells carrying pCRTEIL exhibit HcRed1 (red fluorescence) but neither EGFP nor luc. However, upon transfection with a Cre-expression vector, they will exhibit EGFP (green fluorescence), because the loxP-flanked sequence in the integrated pCRTEIL transgenes is removed by transiently-expressed Cre protein. Thus, the behavior of descendants of the cells exhibiting Cre-mediated gene activation, which will exhibit EGFP continuously, can be monitored in real-time in vivo and in vitro.
2. Materials and Methods
2.1. Plasmid Construction
The reporter plasmid pCRTEIL (Figure 1(a)) was constructed through several cloning steps. First, pCRT-17, an intermediate product for pCRTEIL, was constructed by inserting an 8.8-kb fragment containing HcRed1 cDNA + poly(A) sites of the SV40 gene isolated from pHcRed1-N1 (Clontech Laboratories, Inc., Palo Alto, Calif, USA) in front of the end of CAT in the pCAG-CAT-lacZ  from which the lacZ gene had already been removed. A DNA fragment containing EGFP cDNA, IRES, and luc cDNA was then placed immediately downstream of the loxP site located at the end of the CAT gene in pCRT-17 to obtain pCRTEIL-6. The resulting pCRTEIL has a backbone of pBluescript SK(-) (Stratagene, La Jola, Calif, USA). The reporter plasmid pCETRIZ (Figure 1(a)) was constructed in a multistep process. First, pACS, an intermediate product carrying “I-Sce I-Sfi I-Xba I-Spe I-Sty I-Nhe I-Sfi I-I-Sce I” cassette, was generated by introducing linker oligo into a pBluescript II-based vector. A fragment containing IRES, lacZ gene, and poly(A) sites was introduced into a Sty I site (which had been blunt) of pACS to generate pACSIZA. Next, pCETIZ was generated by ligation of a 4.5-kb Spe I fragment containing CAG promoter, floxed EGFP cDNA and CAT gene, and poly(A) sites into an Xba I site of pACSIZA. Finally, pCETRIZ was constructed by introducing PCR-amplified HcRed1 cDNA into a Spe I site of pCETIZ.
For expression of Cre, we used pCAG/NCre plasmid (; see Figure 1(b)). pCE-29 plasmid  was also used as a positive control vector for expression of EGFP in murine cells (Figure 1(b)). pCL was constructed by inserting luc cDNA downstream of the CAG promoter (Figure 1(b)) and used as a positive control vector for expression of luc.
2.2. Cell Line and Transfection
NIH3T3 cells were first seeded onto gelatin-coated 6-well dishes (number 4810-020; Iwaki Glass Co., Tokyo, Japan) at a density of 106 cells/well one day before transfection and grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen Co., Carlsbad, Calif, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen Co.) at 37 in an atmosphere of 5% C in air at 37. For transfection of a single plasmid, four g of plasmid DNA was mixed with 8 l of LF2000 (number 11668-027; Invitrogen Co.) in Dulbecco’s modified phosphate-buffered saline without Ca2+ and Mg2+, pH 7.4 (PBS(-)), and a total of 100 l solution was prepared according to the manufacturer’s protocol. For cotransfection, two plasmids (3 g for each) were mixed with 12 l of LF2000 in PBS(-). These DNA/liposome complexes were added to the cell culture and incubated for 1 day at 37. After transfection, cells were observed for fluorescence, as described in what follows.
2.3. Histological Analysis
Cells and tissue sections were observed using an Olympus BX60 fluorescence microscope (Olympus, Tokyo, Japan) with DM505 filters (BP460-490 and BA510IF; Olympus) and DM600 filters (BP545-580 and BA6101F; Olympus), which were used for EGFP and HcRed1 monitoring, respectively. For detection of fluorescence in dissected oviducts, an Olympus BX40 dissecting microscope was used. Microphotographs were taken using a digital camera (FUJIX HC-300/OL; Fujifilm, Tokyo, Japan) attached to the fluorescence microscope and printed out using a Mitsubishi digital color printer (CP700DSA; Mitsubishi, Tokyo, Japan).
2.4. In Vivo Gene Transfer
Intraoviductal injection was performed as described previously . One l of solution containing plasmid DNA and trypan blue (TB; 0.05% final concentration) was slowly injected with a glass micropipette, which had been attached to a mouthpiece, into the ampulla of an oviduct of B6C3F1 females (4- to 6-week-old; purchased from CLEA Japan, Inc., Tokyo, Japan). The DNA introduced per oviduct was pCE-29 (0.2 g), pCRTEIL (0.2 g), pCAG/NCre (0.2 g), pCRTEIL (0.2 g) + pCAG/NCre (0.2 g), or pCL (0.2 g). In each transfection group, 0.02 g of phRL-SV (Promega Co., Madison, Wis, USA) was included as a control for normalization of luc activity as noted in what follows. After finishing the injection, the micropipette was rapidly removed. The oviductal regions were then subjected to in vivo EP. Eight square-wave pulses with pulse duration of 50 milliseconds and electric field intensity of 50 V were administered from a square-wave pulse generator (T820; BTX Genetronics, Inc., San Diego, Calif, USA). One day after in vivo EP, the oviducts were subjected to observation for fluorescence and then to lysis for measurement of luc activity.
Oviducts from the mice receiving plasmid DNA were fixed in 4% paraformaldehyde (PFA) in PBS(-) for 2 days at 4, then immersed in 30% sucrose in PBS(-) for more than 2 days at 4, embedded in Tissue-Tek OCT compound (Sakura Finetek Co., Tokyo, Japan), and finally frozen before serial cryostat sectioning (10–30 m in thickness). Sections were mounted in VectraShield mounting medium (Vector Laboratories, Burlingame, Calif, USA) and observed for fluorescence.
To deliver DNA-coated gold particles to the abdominal skin of adult ICR females in vivo, we used the Helios gene gun (Bio-Rad Laboratories, Hercules, Calif, USA). Before the introduction of DNA, ether-anaesthetized mice were shaved in the abdominal area. Transfection particles were made per Helios gene gun protocol giving a microcarrier loading quantity of 0.5 mg per shot and a DNA loading ratio of 2 or 4 g DNA/mg gold particles (1.0 m). For single transfection with pCETRIZ (control), 1 g of DNA was used. For cotransfection using pCETRIZ plus pCAG/NCre (experiment), 1 g of each DNA was used. Mouse abdominal skin was bombarded with the DNA-coated gold particles using the gene gun pressurized by a helium tank at 300 psi. One bombardment was carried out per mouse using 6 mice. In total, 3 transfections were performed for each group. Animals were killed at 48 hours after gene gun bombardment.
Mouse skin was excised and immediately subjected to observation for fluorescence under UV illumination. The samples were then fixed with 4% PFA in PBS(-) for 1 day at 4. Subsequently, the skin was washed in PBS(-) twice and stained with X-Gal staining solution (X-gal Staining Assay Kit, Genlantis, Calif, USA) at 30 for 24 hours. The stained samples were then washed twice in PBS(-) and observed for lacZ activity under light.
2.5. Assay for Luciferase Activity
Luc assay was performed using a kit (Dual-Luciferase Reporter Assay System (number E1910); Promega Co.). The oviducts isolated 1 day after in vivo EP were homogenized in 1 mL of 1 X reporter lysis buffer (Promega Co.). After centrifugation at 15 000 rpm for 10 minutes at 4, the supernatant (200 l) was transferred to a fresh Eppendorf tube. Cells 1 day after transfection were collected with a cell scraper and precipitated after brief centrifugation. Cell pellets were then lysed with 1 mL of 1 X reporter lysis buffer. Relative light units (RLUs) obtained with luc were measured for 5 seconds following a 2-second delay after the addition of the lysate (10 l) to 50 l of luc assay substrate (Promega Co.) using a luminometer (number TD-20/20; Turner Designs Instrument, Sunnyvale, Calif, USA). RLUs were normalized to micrograms of tissue protein added in the luc assay. Tissue protein determinations were performed using Bradford reagent (Bio-Rad Laboratories, Inc., Hercules, Calif, USA). At least four oviducts were tested in each transfection group, and values were presented as means ± standard deviation (SD).
2.6. Statistical Analysis
Results are presented as means ± SD of separate experiments. The RLU obtained from transfection into oviductal epithelium was analyzed using the SPSS 9.0/PC statistics package (SPSS Inc., Chicago, Ill, USA). Results were compared using one-way analysis of variance (ANOVA) following arcsin transformation of the proportions. The Duncan new multiple range test or Tukey test was performed if ANOVA revealed significant differences among compared values.
Successful in Vitro Cre-Mediated Recombination Results in Switching of Gene Expression
In a preliminary test, we first examined what percentage of single cells can incorporate two constructs simultaneously; NIH3T3 cells were cotransfected with pCRTEIL and pCE-29 by the lipoplex method. One day after transfection, fluorescent cells were inspected under a fluorescence microscope. Eight percent (12/142) and 10% (14/142) of cells exhibited EGFP-derived green and HcRed1-derived red fluorescence, respectively, (data not shown). Twenty-two percent (31/142) of cells expressed both types of fluorescence (data not shown). It was thus estimated that in approximately 20% of single cells, incorporation of two constructs into a single cell occurs at the same time. To test whether Cre-mediated excision of the floxed HcRed1/CAT sequence in the pCRTEIL construct results in generation of EGFP fluorescence (Figure 1(a)), NIH3T3 cells were cotransfected with pCRTEIL and pCAG/NCre by the lipoplex method. One day after transfection, some (9% (10/112)) of these cells exhibited both red and green fluorescence (indicated by arrowheads in Figures 2(d)–2(f)), suggesting incomplete Cre-mediated recombination in the introduced pCRTEIL construct (Table 1). There were a few cells (4% [4/112]) preferentially expressing green fluorescence (indicated by arrows in Figures 2(d)–2(f)), suggesting the occurrence of complete Cre-mediated recombination in the transfected cells (Table 1). Twenty-four percent (27/112) of cells (Table 1) expressed only red fluorescence, suggesting incorporation of two constructs at the same time in a single cell (though gene switching failed) or incorporation of pCRTEIL alone. Considering that 20% of cells can incorporate two constructs simultaneously, the rate of Cre-mediated recombination in this system appears to be greater than 50% at a minimum. In the control experiment in which cells were transfected with pCRTEIL alone, some cells (35% (36/102); Table 1) exhibited HcRed1-derived red fluorescence but not EGFP-derived green fluorescence (arrows in Figures 2(b) versus 2(c)).
Similar results were also obtained when pCETRIZ was transfected singly or cotransfected with pCAG/NCre (Figures 2(g)–2(n)). In this system, expression of lacZ protein arises from the recombined form of pCETRIZ together with HcRed1 after Cre-mediated recombination. In fact, cells expressing HcRed1 exhibited lacZ, which could be visualized after fixation of cells and subsequent staining with X-Gal (arrows in Figures 2(k)–2(n)). Transfection of cells with a single pCETRIZ resulted in expression of EGFP but not of both HcRed1 and lacZ (arrows in Figures 2(g)–2(j)). These findings indicate that pCRTEIL and pCETRIZ as reporter plasmids work well with cultured murine cells.
Successful in Vivo Cre-Mediated Recombination in pCRTEIL System
We next examined whether this Cre-loxP system using pCRTEIL as a reporter plasmid can also be used in vivo. For this purpose, gene delivery into oviductal epithelium was performed by DNA injection into the lumen of oviducts and subsequent EP . Instillation of pCE-29 plasmid yielded bright green fluorescence throughout the ampulla ((b) in Figure 3(A)) but not red fluorescence ((c) in Figure 3(A)). When pCRTEIL plasmid DNA was singly introduced, no fluorescence for EGFP was observed ((e) in Figure 3(A)). Instead, red fluorescence was observed in some oviductal epithelial cells ((f) in Figure 3(A)). Coinjection with pCRTEIL and pCAG/NCre plasmids resulted in generation of both red and green fluorescence, although green fluorescence appeared to be weaker than red fluorescence (indicated by arrows in (h) and (i) in Figure 3(A)).
To examine the localized expression of EGFP and HcRed1 in the oviducts after cotransfection, cryostat sections were prepared and inspected for EGFP and red fluorescence. Strong, but patchy, EGFP fluorescence was observed in some oviductal epithelial cells (indicated by arrows in (k) in Figure 3(A)), together with weak red fluorescence throughout the EGFP-positive area (data not shown). These findings indicate that the Cre-loxP system using pCRTEIL works well even in vivo, and as expected HcRed1 exhibits a nonoverlapping spectral profile from EGFP.
The IRES sequence is present between EGFP and luc cDNAs in the pCRTEIL plasmid (Figure 1(a)). Therefore, the two proteins (EGFP and luc) should be produced at the same time in a cell into which pCRTEIL and pCAG/NCre have been cotransfected, since an IRES sequence allows cap-independent translation initiation [10, 11] from the dicistronic mRNA generated. In fact, we observed increase in luc activity repeatedly when oviductal epithelial cells were cotransfected with pCRTEIL and pCAG/NCre (Figure 3(B)). This finding also suggests successful in vivo Cre-mediated recombination.
Successful in Vivo Cre-Mediated Recombination in pCETRIZ System
Cells carrying pCETRIZ are expected to exhibit HcRed1 and lacZ after Cre-mediated excision. For testing the feasibility of pCETRIZ-based Cre-loxP system in vivo, tissues that never exhibit endogenous -galactosidase activity must be chosen. In this occasion, skin epidermis appeared to be suitable for this purpose, because it does not exhibit such kind of activity  and has been considered as an attractive target to study gene delivery due to its easy accessibility and visualization . Since particle-mediated devices have been studied to deliver genes to skin efficiently [12, 14, 15], we introduced pCETRIZ (control) or pCETRIZ + pCAG/NCre (experiment) into mouse skin using Helios gene gun. Skin biopsies were taken after 48 hours for inspection of fluorescence and lacZ staining. In the samples bombarded with single pCETRIZ, green fluorescence was detected over the area gene-transfected (Figure 4(b)). However, neither red fluorescence nor lacZ activity was noted in the area exhibiting green fluorescence (Figures 4(c) and 4(d)). This was also observed in the other 2 injected mice. Samples bombarded with pCETRIZ + pCAG/NCre showed coexpression of green and red fluorescence (arrows in Figures 4(f) and 4(g)), suggesting successful Cre-mediated excision of the introduced pCETRIZ. As expected, lacZ staining revealed expression of lacZ over the area cotransfected (Figure 4(h)). These patterns of gene expression were also seen in the other 2 injected mice.
The Cre-loxP system permits the generation of mouse models, in which the fate of a cell can be followed through time. In such models, a cell-type-specific promoter that is turned on at a given stage of differentiation regulates Cre-expression. When combined with a reporter system, regulated expression of Cre may lead to specific expression of inductive reporter molecules and permanent tagging of those cells, which have completed a defined genetic program. An essential component of such strategy is the development of appropriate reporter strains of mice in which the inducible reporter molecule is ubiquitously expressed.
Several reporter strains carrying different floxed transgenes and exhibiting switching of gene expression upon Cre-mediated recombination have been reported in mice (i.e., CAG-CAT-Z , Rosa26R (R26R) , Z/AP , Z/EG , ROSA26flox , CMV-floxed stop-lacZ , ACZL , and CETZ-17 ). Almost all have employed a single fluorescent marker gene, alkaline phosphatase, (ALP) and/or lacZ gene. The most commonly utilized strain appears to be Rosa26R mice , which are generated by targeting a reporter construct to the proviral ROSA26 locus that is constitutively expressed in all tissues. In these reporter strains carrying lacZ or ALP, detection of these reporters following Cre-mediated recombination should always be performed for fixed samples, thus hampering continuous observation of activated gene products in living cells or embryos. There are only a few reports on reporter strains capable of exhibiting noninvasive fluorescent markers upon Cre-mediated excision of a “stop sequence.” Such strains carry a single fluorescent marker (EGFP for Schwenk et al. , Mao et al. , Motoike et al. , and Kawamoto et al. , DsRed-T3 for Vintersten et al. , and ECFP or EYFP for Srinivas et al. ), placed downstream of the floxed stop sequence. In our CRTEIL system, the reporter plasmid contains two fluorescent markers (i.e., floxed HcRed1 cDNA and EGFP placed downstream of the floxed gene) enabling monitoring of gene switching based on changes from red to green fluorescence (see Figure 1(a)). This type of Cre excision reporter construct was first reported by Yang and Hughes , who adopted two fluorescent markers (EGFP and DsRed1 in their reporter plasmid, called Cre stoplight) and demonstrated that cells carrying Cre stoplight as transgene can be converted from red to green by transient expression of Cre. However, DsRed1 protein has displayed toxicity in murine embryos . For these reasons, we used HcRed, a dimeric far-red fluorescent protein variant  isolated from the coral reef Heteractis crispa, which exhibits a spectral profile (exc. 588 nm, em. 618 nm) that does not overlap with those of other existing fluorescent proteins. HcRed1 has already been used as a safe noninvasive protein marker in a transgenic system .
Recently, Muzumdar et al.  generated mT/mG; a double-fluorescent Cre reporter mouse that expresses membrane-targeted tandem dimer Tomato (mT) prior to Cre-mediated excision and membrane-targeted green fluorescent protein (mG) after excision. The use of a double-fluorescent marker system by Muzumdar et al.  is the same as ours. They observed that this strain works well in vivo after mating with the pre-existing Cre expressor strains. This encouraged us to generate transgenic mouse strains carrying CRTEIL/CETRIZ transgenes. The advantage of our system over that of Muzumdar et al. , may be the employment of IRES-luc and IRES-lacZ. These elements will be useful when tissue samples are after Cre-mediated excision subjected to biochemical and histochemical analyses, since we can measure the level of luc or lacZ quantitatively using commercially available kits (e.g., the Luminescent -galactosidase Detection Kit II (Clontech Laboratories, Inc., Palo Alto, Calif, USA)) after observation for fluorescence. In this study, ca. 630-bp IRES placed between EGFP and luc sequences was useful for simultaneous expression of the two proteins, though the level of luc placed downstream of the IRES appeared to be greatly decreased (approximately 1/400 of luc activity in the oviducts transfected with pCL alone; see Figure 3(B)). This appears to be due to the insertion of IRES between EGFP and luc, since the level of luc in the oviducts transfected with pCTL (composed of CAG promoter, loxP-flanked CAT sequence, and luc gene) + pTC (carrying an NCre gene controlled by Herpes simplex virus-derived thymidine kinase promoter) was only 1/4 of that in the oviducts transfected with pCL alone . In this sense, use of 81-bp 2A sequence, which is known to yield synthesis of two different proteins from a single mRNA [34, 35], may be appropriate in our system, since similar levels of each of the proteins have been reported to be produced in cells transfected with the 2A-containing construct .
In this study, we have described in vitro and in vivo switching of gene expression from red to green or green to red fluorescence by the Cre-loxP system when pCRTEIL and pCETRIZ plasmids are used as tester plasmids. These events occurred when tester plasmid and Cre-expression plasmid are cotransfected (see Figures 2–4). In our LF2000-based transfection system, approximately 20% of NIH3T3 cells could incorporate two constructs simultaneously, of which more than 50% exhibited Cre-mediated gene switching. This rate appears to be too low, since in our previous experiments using F9 embryonal carcinoma cell line stably transfected with loxP-containing reporter plasmid pCETZ-17, all clones exhibited gene switching within one day after transient expression of pCAG/NCre . This means that 100% gene switching is possible if stable cell lines carrying a reporter construct are subjected to Cre-mediated transfection. The low efficiency in Cre-mediated recombination in the present study may be due solely to the cotransfection method itself, since we cannot strictly control the number of copies of exogenous gene introduced into cells.
The present Cre-loxP system based on two fluorescent markers appeared to have great advantages over the previous systems, since the process of change in gene expression can be easily monitored in noninvasive fashion. Furthermore, it is possible to trace the fate of the descendants that express fluorescence under physiological conditions. For finer analysis of the fate of cells transfected with exogenous Cre-expression vector, it will be convenient to use transgenic mouse lines carrying the CRTEIL or pCETRIZ transgene as generalized reporter strains. This experiment is now underway.
The authors would like to thank Dr. Kimi Araki (Kumamoto University, Japan) for allowing them to use pCAG-CAT-Z plasmid. They also thank Kazunori Kiryu and Masaki Takeda for their support on cryostat-sectioning. This study was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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