Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing
Figure 3
Analysis of IFN-γ expression in transduced DC and T cells by RT-PCR. Cells were infected, mRNA isolated and analyzed by RT-PCR as described in Section 2. (a) shows the results from DC. (b) shows the results from T cells. Note that both AAV/IFN-γ transduced DC and T cells demonstrate IFN-γ expression. Also note that untransduced T cells expressed some IFN-γ, but that AAV/IFN-γ transduced T cells demonstrate higher IFN-γ expression. This is confirmed in Figure 4.
