Figure 3: DIGE analysis of normal versus dystrophic MDX cardiac muscle. Shown are Cy5-labelled master gels of the soluble fraction from mouse heart muscle, covering both the pH 4–7 (a) and pH 6–11 (b) range. Protein spots with a drastically different expression level are marked by circles and are numbered 1 to 79. See Table 1 for a detailed listing of cardiac muscle proteins with a changed abundance in dystrophin-deficient tissue. The pH-values of the first dimension gel system and molecular mass standards (in kDa) of the second dimension are indicated on the top and on the left of the panels, respectively.