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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 705215, 15 pages
Review Article

Induction/Engineering, Detection, Selection, and Expansion of Clinical-Grade Human Antigen-Specific Cytotoxic T Cell Clones for Adoptive Immunotherapy

1Tissue Typing Center, Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia
2Cell Engineering Laboratory, Celica, Biomedical Center, Technology Park 24, 1000 Ljubljana, Slovenia
3Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia

Received 5 December 2009; Accepted 28 January 2010

Academic Editor: Zhengguo Xiao

Copyright © 2010 Matjaž Jeras et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Adoptive transfer of effector antigen-specific immune cells is becoming a promising treatment option in allogeneic transplantation, infectious diseases, cancer, and autoimmune disorders. Within this context, the important role of cytotoxic T cells (CTLs) is objective of intensive studies directed to their in vivo and ex vivo induction, detection, selection, expansion, and therapeutic effectiveness. Additional questions that are being addressed by the scientific community are related to the establishment and maintenance of their longevity and memory state as well as to defining critical conditions underlying their transitions between discrete, but functionally different subtypes. In this article we review and comment latest approaches and techniques used for preparing large amounts of antigen-specific CTLs, suitable for clinical use.