Figure 3: Reduction in titin-based tension (a) and titin-based stiffness (b). Data are based on measurements shown in Figure 2 (tension) and from the slope of the tension-SL curve shown in Figure 2 (stiffness). The reductions in tension and stiffness are SL dependent, in all tissue types, and are highest in MLV, lowest in BLA and intermediate in BLV. Error bars are SE (for clarity plotted only every 0.05  𝜇 m).