BioMed Research International / 2010 / Article / Fig 2

Research Article

Reduction of Foxp Cells by Depletion with the PC61 mAb Induces Mortality in Resistant BALB/c Mice Infected with Toxoplasma gondii

Figure 2

Kinetics analysis of the depletion of CD4+CD25+ cells. BALB/c mice were injected ip with 100  πœ‡ g ((a)–(e)) or 200  πœ‡ g ((f)–(j)) of PC61 mAb. Spleen cells were obtained at different time points and immunofluorescence was carried out as described in Figure 1 using anti-CD4-FITC and anti-CD25-APC mAbs. The lymphocyte region was first identified and gated by FSC and SSC characteristics, the CD4+ region was subgated and 10,000 CD4+ events were captured. Numbers indicate % of CD4+CD25+ cells within the CD4+ population.
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