BioMed Research International / 2010 / Article / Fig 5

Research Article

Reduction of Foxp Cells by Depletion with the PC61 mAb Induces Mortality in Resistant BALB/c Mice Infected with Toxoplasma gondii

Figure 5

Analysis of Tregs and Tact cells after PC61 injection and infection with T. gondii. Animals were depleted and infected as described in Figure 4. At 10 dpi spleens were obtained and immunofluorescence was carried out using anti-CD4-PerCP, anti-Foxp3-Alexa Fluor 488 and anti-CD25-PE mAb, either PC61 (a)–(d) or 7D4 clone (e)–(h). The lymphocyte region was first identified and gated by FSC and SSC characteristics, the CD4+ region was subgated, and 10,000 CD4+ events were captured. Numbers indicate % of cells from the CD4+ gating. A representative analysis from one mouse per group is shown.

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