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Journal of Biomedicine and Biotechnology
Volume 2010 (2010), Article ID 832341, 8 pages
Methodology Report

Proposing Low-Similarity Peptide Vaccines against Mycobacterium tuberculosis

Department of Biochemistry and Molecular Biology “Ernesto Quagliariello”, University of Bari, Bari 70126, Italy

Received 23 September 2009; Revised 2 December 2009; Accepted 24 March 2010

Academic Editor: Yongqun Oliver He

Copyright © 2010 Guglielmo Lucchese et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Using the currently available proteome databases and based on the concept that a rare sequence is a potential epitope, epitopic sequences derived from Mycobacterium tuberculosis were examined for similarity score to the proteins of the host in which the epitopes were defined. We found that: (i) most of the bacterial linear determinants had peptide fragment(s) that were rarely found in the host proteins and (ii) the relationship between low similarity and epitope definition appears potentially applicable to T-cell determinants. The data confirmed the hypothesis that low-sequence similarity shapes or determines the epitope definition at the molecular level and provides a potential tool for designing new approaches to prevent, diagnose, and treat tuberculosis and other infectious diseases.