Proposing Low-Similarity Peptide Vaccines against Mycobacterium tuberculosis

Figure 1

Similarity profile of the mycobacterial DNAK carboxy-terminus domain versus the human proteome at the pentapeptide level. The columns indicate the number of DNAK pentapeptide occurrences in the human proteome. DNAK corresponds to hsp70 protein, UniProt accession: P0A5B9; length: 609 aa. The numbered arrows indicate the location of mycobacterial peptide sequences immunoassayed with human sera [41]. Black arrows refer to the immuno-negative peptide sequences corresponding to IEDB ID: (6) 1212577 (aa FVKEQREA); (7) 1212579 (aa VKEQREAE); (8) 1212581 (aa KEQREAEG); (9) 1212582 (aa EQREAEGG); (10) 1212585 (aa QREAEGGS); (11) 1212586 (aa REAEGGSK); (12) 1212589 (aa EAEGGSKV); (13) 1212590 (aa AEGGSKVP); (14) 1212593 (aa EGGSKVPE); (15) 1212595 (aa GGSKVPED). Red arrows refer to the positive epitopic sequences corresponding to IEDB ID: (1) 1052559; (2) 1052560; (3) 1052558; (4) 1052561; (5) 1052562; (16) 1052583; (17) 1052584; (18) 1052589; (19) 1052591; (20) 1052593; (21) 1052594; (22) 1052595; (23) 1052596; (24) 1052597; (25) 1052598; (26) 24658; (27) 1052627; (28) 1052628; (29) 1052629; (30) 1052630; (31) 1052631; (32) 1052632; (33) 1052633; (34) 1052549; (35) 1052550. See Table 1 for amino acid sequence details. IEDB ID Reference Dataset of Mycobacterial Immune Epitopes: http://iedb.zendesk.com/entries/18171-reference- datasets-of-mycobacterial-immune-epitopes.